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Preparation method of multivalence egg-yolk immunoglobulin for resisting diarrheas

A technology for yolk immunoglobulin and diarrhea, which is applied in the field of preparation of anti-diarrhea polyvalent yolk immunoglobulin, can solve the problems of single anti-diarrheal function, monovalent, narrow anti-diarrheal range, etc., and achieves low cost, high yield, and recovery. high rate effect

Inactive Publication Date: 2010-12-22
SHANGHAI WANWUCHUN BIO TECH
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Problems solved by technology

[0005] The researches on anti-diarrhea yolk immunoglobulin (IgY) have been reported so far, the IgY is monovalent, the function of anti-diarrhea is relatively single, and the scope of anti-diarrhea is relatively narrow

Method used

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Embodiment

[0027] A method for preparing anti-diarrhea multivalent yolk immunoglobulin, the method comprising the following steps:

[0028] (1) Specific immunity of laying hens: using immunological methods, Escherichia coli (A, B and C) and human rotavirus (HRV) are made into vaccines to immunize laying hens at the same time. High-titer specific antibodies (titer>1:256) are produced in chicken serum and egg yolk, and can be maintained for about 35-50 days without significant reduction. When the antibody titer in eggs has decreased to a large extent, the titer can be restored to the highest level by supplementary immunization, and it can last for about 50 days. In addition, there is a certain synergistic effect when multiple vaccines are immunized at the same time. Eggs were collected to prepare multivalent yolk immunoglobulin (IgY) with antidiarrheal function, and its effect was verified. Specific immunization time:

[0029] Basic immunization: 19-week-old laying hens, 1ml / feather ino...

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Abstract

The invention relates to a preparation method of multivalence egg-yolk immunoglobulin for resisting diarrheas, comprising the following steps of: (1) laying hen immunization: simultaneously immunizing laying hens by using a vaccine prepared from Escherichia coli and human rotaviruses in an immunology method; and (2) separation and purification of the multivalence egg-yolk immunoglobulin for resisting the diarrheas: degreasing the yolks of eggs collected in the step (1) in a water dilution method to obtain a multivalence egg-yolk immunoglobulin solution with the function of diarrhea resistance, concentrating the solution by 4-8 times by using 5 thousand membranes, then salting out the concentrated solution at the ammonium sulfate saturation of 45%-50% and the pH value of 6.0-7.0, sequentially ultrafitering the dissolved sediment by using 50 thousand membranes and 5 thousand membrane, carrying out chromatographic treatment to the salted and ultrafiltered product and then further purifying the product to obtain the multivalence egg-yolk immunoglobulin. Compared with the prior art, the preparation method has the advantages of low cost, high yield, wide application range and the like.

Description

technical field [0001] The invention relates to egg yolk immunoglobulin, in particular to a preparation method of anti-diarrhea multivalent egg yolk immunoglobulin. Background technique [0002] Chicken yolk immunoglobulin G antibody is a 7S immunoglobulin that is slightly different from mammalian IgG. The protein has a molecular weight of about 180kDa and contains two subunits, namely a heavy chain of 67-70kDa and a light chain of 22-30kDa, also known as IgY (Yolk Immunoglobulin), present in the yolk of the immunized hen. Experiments have shown that after immune stimulation, the hen produces an immune response. During the yolk maturation period in the oviduct, IgG in the blood can be selectively transferred to the yolk, and it is the only immunoglobulin in the yolk. As early as the 1960s, scientists discovered that IgG antibodies existed in chicken yolk, and its content was similar to or even higher than that of chicken serum, but it has not attracted enough attention. St...

Claims

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Application Information

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IPC IPC(8): C07K16/12C07K16/10C07K16/02C07K1/14A61P1/12
Inventor 伍勤观阮莲华
Owner SHANGHAI WANWUCHUN BIO TECH
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