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OBR (obese receptor) gene and application thereof as goose fat traits genetic markers

A technology of genetic markers and genes, applied in related fields, can solve the problems of no goose OBR gene research reports, few chickens, etc., and achieve significant application value and economic benefits

Inactive Publication Date: 2010-12-22
曲湘勇 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are few studies on DNA genetic markers, genetic diversity and related functional genes of geese compared with chickens, and there is no research report on goose OBR genes

Method used

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  • OBR (obese receptor) gene and application thereof as goose fat traits genetic markers
  • OBR (obese receptor) gene and application thereof as goose fat traits genetic markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Goose related gene SNP screening

[0026] Sixty-five healthy young Xupu geese and 130 Sichuan white geese were selected for the feeding experiment. All tested geese were slaughtered and measured at the age of 84 days, and the measurement and calculation were carried out in accordance with the "Poultry Production Performance Terminology and Measurement and Statistical Methods" in the "Technical Manual for Investigation of Livestock and Poultry Genetic Resources". Use electronic scales to measure carcass weight, eviscerated weight, liver weight, abdominal fat weight, and measure sebum thickness with calipers. Calculate the carcass fat trait ratio index.

[0027] After 12 hours of fasting for 60 Xupu geese and 120 Sichuan white geese that are about to be slaughtered and measured, 1ml of blood was collected from the subwing vein with a disposable medical syringe (2.5ml) and placed in a 5ml centrifuge tube, anticoagulated with 75% ethanol , refrigerated at 4°C fo...

Embodiment 2

[0045] Example 2 Verification of SNPs related to abdominal fat rate in production practice

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Abstract

The invention relates to an OBR (obese receptor) gene and application thereof as goose fat traits genetic markers. Genetic markers related to abdomen fat weight, abdomen fat ratio and blood plasma VLDL (Very Low Density Lipoprotein) concentration in the OBR gene are measured; the existence of polymorphism in the OBR gene in a nucleic acid sample obtained from a goose is measured, wherein the polymorphism is related to the abdomen fat weight, the abdomen fat ratio and the blood plasma VLDL concentration; and the fat traits selection breeding can be carried out on the goose containing the OBR gene in a genome DNA according to the single nucleotide polymorphism of the gene, and an advantageous genotype for reducing the abdomen fat weight, the abdomen fat ratio and the blood plasma VLDL concentration is obtained by the screening. Therefore, the invention has extremely important application value and economic benefit.

Description

Technical field: [0001] The present invention relates to the field of molecular biology technology and breeding of geese, in particular to the determination of the presence of genetic markers associated with characteristics such as abdominal fat weight, abdominal fat percentage and plasma VLDL concentration, and more specifically to the determination of the presence of these characteristics The difference of the OBR gene, and further specifically, is to determine the polymorphism in the OBR gene, that is, the single nucleotide polymorphism of the goose OB (obese) gene related to abdominal fat weight, abdominal fat rate and plasma VLDL concentration Sex (Single nucleotide polymorphism, abbreviated as SNP) exists. Background technique: [0002] The development of modern poultry genetic breeding theory and application has greatly improved the growth rate and feed efficiency of broiler poultry, especially broiler chickens and ducks, and the production efficiency has gradually ad...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12Q1/68
Inventor 曲湘勇何俊蒋隽
Owner 曲湘勇
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