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Lyssa virus diagnosing and typing chip and manufacturing method thereof

A rabies virus and chip technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of long test period, cumbersome technology, low sensitivity, etc., and achieve simple operation, good repeatability and high sensitivity. Effect

Inactive Publication Date: 2010-12-22
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0006] The invention discloses a lyssavirus diagnosing and typing chip and a method for preparing the chip, a gene chip (or oligonucleotide microarray) used for lyssavirus diagnosing and typing, which overcomes the existing Lv diagnosis and typing The method has the disadvantages of low sensitivity, cumbersome technology, high cost and long test period

Method used

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  • Lyssa virus diagnosing and typing chip and manufacturing method thereof
  • Lyssa virus diagnosing and typing chip and manufacturing method thereof
  • Lyssa virus diagnosing and typing chip and manufacturing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Production of Gene Chips

[0043] Gene chip production materials include: optical-grade aldehyde-modified glass substrate, spotting solution, seven genotype-specific probes for Lv, control probes and positioning probes;

[0044](1) Optical-grade aldehyde-modified glass substrate and spotting solution;

[0045] Crystal core optical grade aldehyde-based substrate, batch number: 20091114 (Boao Biotechnology Co., Ltd., Beijing); crystal core gene chip sample solution, batch number: 20090501 (Boao Biotechnology Co., Ltd., Beijing);

[0046] (2) Design and synthesis of Lv genotype-specific probes

[0047] Probe design: According to the full gene sequence and N gene sequence of each genotype of Lv registered in the GenBank database, the main sequence of each type N gene was obtained by using the bioinformatics software Lasergene 7.0 analysis, located in the 55bp of the N gene coding region of Lv Oligonucleotide probes were designed between ~899bp (the homology between th...

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Abstract

The invention discloses a lyssa virus (Lv) diagnosing and typing chip and a manufacturing method thereof, wherein the chip is a gene chip (or an oligonucleotide microarray) for Lv diagnosis and typing, and the method can simultaneously detect and type seven genotypes of Lv in a clinical sample and has the characteristics of rapidness, sensitivity, strong specificity, high accuracy and the like.

Description

technical field [0001] The invention discloses a lyssavirus diagnosis and typing chip and a chip preparation method, a gene chip (or oligonucleotide microarray) used for lyssavirus diagnosis and typing, and belongs to the field of biotechnology. Background technique [0002] Rabies is a zoonotic disease to which all warm-blooded animals are susceptible, and the pathogen of rabies belongs to Lv. Lv belongs to Rhabdoviridae ( Rhabdoviridae ) Unimolecular negative-strand RNA viruses ( Mononegavirales ), highly neurotropic, often causing fatal encephalomyelitis in humans and animals. Lv mainly includes 7 genotypes, namely: classic rabies virus (RABV) genotype 1; Lagos bat virus (LBV) genotype 2; Mokola virus (MOKV) genotype 3; Duvenhage virus genotype 4 (DUVV); genotype 5 European bat lyssa virus 1 (EBLV-1); European bat of genotype 6 lyssa virus 2 (EBLV-2); Australian bat of genotype 7 lyssa virus (ABLV). At present, the field isolates of human and animal rabies in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 涂长春席进
Owner MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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