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Method for separating and detecting purple sweet potato haematochrome effective component

The technology of purple-fleshed sweet potato and effective components is applied in the field of medicine and health care, which can solve the problems of low yield of raw materials, and achieve the effects of easy quality control and reasonable design.

Inactive Publication Date: 2010-12-22
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the yields of these raw materials are very low

Method used

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  • Method for separating and detecting purple sweet potato haematochrome effective component
  • Method for separating and detecting purple sweet potato haematochrome effective component
  • Method for separating and detecting purple sweet potato haematochrome effective component

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Reversed-phase silica gel conventional column filling: Weigh 50g of reverse-phase silica gel with a particle size of 10-15um in 50ml of analytical pure methanol, mix well, and slowly pour it into a diameter-to-height ratio of 1:8-1:15 (such as In a chromatographic column with a diameter of 2.5 cm and a length of 20 cm), the flow rate of 1 ml per minute is controlled by the valve of the chromatographic column, and methanol flows out. Tap the chromatography column with a rubber tube to let the silica gel evenly deposit and fill it into the column. When the liquid level is about 4cm higher than the silica gel surface, close the valve, pressurize with a small air pump, and continue to tap the silica gel chromatography column to let the silica gel The post bed is flat and solid. The height of the silica gel column is controlled at 15cm.

[0021] (2) Reversed-phase silica gel column mobile phase equilibration: Use 3 times column volume containing 20% ​​methanol and 3% fo...

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Abstract

The invention provides a method for separating and detecting a purple sweet potato haematochrome effective component. The total purple sweet potato haematochrome is separated into 11 high-purity main components or monomers by reverse phase silica gel step-by-step chromatographic separation, elution and concentration for drying. HPLC (High Performance Liquid Chromatography) analysis is carried out on the 11 components, the component purity is calculated, and the oxidization resistant activity of the components is tested by utilizing an in-vitro oxidization resistant test. The component purity is analyzed according to an HPLC method, the purity of a main compound in the components is 40-99.9 percent, and the in-vitro oxidization resistant activity capability test is evaluated by utilizing four systems of total reducing capacity, DPPH free radical inhibition, hydroxyl free radical inhibition and linoleic acid lipid peroxidation inhibiting capability. The purple sweet potato haematochrome effective component provided by the invention can be applied to preparing oxidization resistant medicines and health-care products and be used as a natural food additive. The method of the invention has reasonable design, can rapidly and accurately obtain the effective component and is easier for the quality control of the medicines or foods in production.

Description

technical field [0001] The invention belongs to the field of medicine and health care, and in particular relates to the preparation of a component with strong antioxidant activity and high purity from total red pigment of purple-fleshed sweet potato and its application in medicines, health products and food additives. Background technique [0002] Food coloring additives currently used in the food industry can be divided into two categories: artificial pigments and natural pigments. Although artificial pigments have various colors, strong coloring, firm coloring, stable properties and low price, all synthetic pigments can only be colored but have no nutritional value, and some are harmful to the human body and even have potential carcinogenic effects, thus limiting the use of artificial pigments and scope. According to reports, there were more than 100 kinds of artificial synthetic pigments in the 1950s in various countries in the world, but as of 2008, only 9 kinds were al...

Claims

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Application Information

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IPC IPC(8): G01N30/89
Inventor 陈敏廖志华付玉凡谌金吾魏文丽
Owner SOUTHWEST UNIVERSITY
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