Application of Soracan gum in reducing liver fat, blood fat, body fat, body weight and cholesterol
A technology for solara gum and cholesterol is applied in the application field of the aspect, can solve the problems of restricting large-scale use, increasing the difficulty of β-glucan, increasing the preparation cost, etc., and achieves safe and non-toxic cholesterol, simple extraction method, and high yield. big effect
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Embodiment 1
[0047] Example 1: Preparation of crude solarala gum
[0048] Prepare the culture medium, NaH 2 PO 4 0.75g, KNO 3 2.5g, CaCl 2 0.07g, MgCl 2 0.25g, FeSO 4 ·7H 2 O0.006g, MnSO 4 0.001g, ZnCl 2 0.006g, NaCl 10g, sucrose 20g, H 2 01000 mL, pH 6. Sterilize the culture solution at 121°C for 20 minutes, cool to 32°C and insert 200 μL of colony solution on a plate, and cultivate at 28°C for 12 hours to obtain a seed solution. 5% seed culture solution was added to the sterilized (sterilized at 121°C for 20 minutes) culture solution and cooled to 32°C, and fermented at 28°C for 24 hours. Two volumes of propanol were added to the fermentation broth to precipitate, and the precipitate was vacuum-dried at 50° C. to obtain crude solarara gum.
Embodiment 2
[0049] Example 2: Preparation of crude solarala gum
[0050] Prepare the culture medium, NaH 2 PO 4 2g, KNO 3 2.5g, CaCl 2 0.14g, MgCl 2 0.45g, FeSO 4 ·7H 2 O 0.0012g, MnSO 4 0.002g, ZnCl 2 0.0065g, NaCl 35g, sucrose 30g, H 2 01000 mL, pH 10. The culture solution was heated at 121°C for 30 minutes, cooled to 36°C, and 100 μL of colony solution on a plate was added, and cultured at 28°C for 24 hours to obtain a seed solution. 10% seed culture solution was added to the sterilized (sterilized at 121°C for 30 minutes) culture solution and cooled to 36°C, and fermented at 28°C for 48 hours. Add twice the volume of 95% ethanol to the fermentation broth to precipitate, and vacuum-dry the precipitate at 70° C. to obtain crude solarara gum.
Embodiment 3
[0051] Example 3: Preparation of crude solarala gum
[0052] Prepare the culture medium, NaH 2 PO 4 1.0g, KNO 3 3g, CaCl 2 0.08g, MgCl 2 0.2g, FeSO 4 ·7H 2 O 0.0075g, MnSO 4 0.003g, ZnCl 2 0.007g, NaCl 60g, sucrose 30g, H 2 O1000mL, pH 7.2, heat the culture solution at 121°C for 25 minutes, cool to 34°C, insert 200μL of colony solution on a plate, and cultivate at 30°C for 36h, which is the seed solution. 7% seed culture solution was added to the sterilized (sterilized at 121°C for 25 minutes) culture solution and cooled to 34°C, and fermented at 28°C for 36 hours. Add twice the volume of 95% ethanol to the fermentation broth to precipitate, and vacuum-dry the precipitate at 60° C. to obtain crude solarara gum.
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