Application of Soracan gum in reducing liver fat, blood fat, body fat, body weight and cholesterol

A technology for solara gum and cholesterol is applied in the application field of the aspect, can solve the problems of restricting large-scale use, increasing the difficulty of β-glucan, increasing the preparation cost, etc., and achieves safe and non-toxic cholesterol, simple extraction method, and high yield. big effect

Active Publication Date: 2010-12-29
SICHUAN HETAI SYNLIGHT BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But all these solutions increase the difficulty of obtaining the required β-glucan, gr...

Method used

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  • Application of Soracan gum in reducing liver fat, blood fat, body fat, body weight and cholesterol
  • Application of Soracan gum in reducing liver fat, blood fat, body fat, body weight and cholesterol
  • Application of Soracan gum in reducing liver fat, blood fat, body fat, body weight and cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of crude solarala gum

[0048] Prepare the culture medium, NaH 2 PO 4 0.75g, KNO 3 2.5g, CaCl 2 0.07g, MgCl 2 0.25g, FeSO 4 ·7H 2 O0.006g, MnSO 4 0.001g, ZnCl 2 0.006g, NaCl 10g, sucrose 20g, H 2 01000 mL, pH 6. Sterilize the culture solution at 121°C for 20 minutes, cool to 32°C and insert 200 μL of colony solution on a plate, and cultivate at 28°C for 12 hours to obtain a seed solution. 5% seed culture solution was added to the sterilized (sterilized at 121°C for 20 minutes) culture solution and cooled to 32°C, and fermented at 28°C for 24 hours. Two volumes of propanol were added to the fermentation broth to precipitate, and the precipitate was vacuum-dried at 50° C. to obtain crude solarara gum.

Embodiment 2

[0049] Example 2: Preparation of crude solarala gum

[0050] Prepare the culture medium, NaH 2 PO 4 2g, KNO 3 2.5g, CaCl 2 0.14g, MgCl 2 0.45g, FeSO 4 ·7H 2 O 0.0012g, MnSO 4 0.002g, ZnCl 2 0.0065g, NaCl 35g, sucrose 30g, H 2 01000 mL, pH 10. The culture solution was heated at 121°C for 30 minutes, cooled to 36°C, and 100 μL of colony solution on a plate was added, and cultured at 28°C for 24 hours to obtain a seed solution. 10% seed culture solution was added to the sterilized (sterilized at 121°C for 30 minutes) culture solution and cooled to 36°C, and fermented at 28°C for 48 hours. Add twice the volume of 95% ethanol to the fermentation broth to precipitate, and vacuum-dry the precipitate at 70° C. to obtain crude solarara gum.

Embodiment 3

[0051] Example 3: Preparation of crude solarala gum

[0052] Prepare the culture medium, NaH 2 PO 4 1.0g, KNO 3 3g, CaCl 2 0.08g, MgCl 2 0.2g, FeSO 4 ·7H 2 O 0.0075g, MnSO 4 0.003g, ZnCl 2 0.007g, NaCl 60g, sucrose 30g, H 2 O1000mL, pH 7.2, heat the culture solution at 121°C for 25 minutes, cool to 34°C, insert 200μL of colony solution on a plate, and cultivate at 30°C for 36h, which is the seed solution. 7% seed culture solution was added to the sterilized (sterilized at 121°C for 25 minutes) culture solution and cooled to 34°C, and fermented at 28°C for 36 hours. Add twice the volume of 95% ethanol to the fermentation broth to precipitate, and vacuum-dry the precipitate at 60° C. to obtain crude solarara gum.

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Abstract

The invention discloses an application of Soracan gum in reducing liver fat, blood fat, body fat, body weight and cholesterol, which comprises the following steps: firstly preparing a culture solution, and heating the culture solution for sterilization; adding a plate colony solution to the cooled culture solution; placing the culture solution in a shaking table for fermentation, and forming the culture solution into a seed culture solution; cooling the obtained culture solution, adding to the obtained the seed culture solution, and fermenting in the shaking table to obtain a viscous metabolite; injecting ethanol or acetone into the obtained fermentation liquid, and depositing, wherein the volume of the ethanol or acetone is at least twice of that of the fermentation liquid; and drying the obtained precipitate in vacuum to obtain a crude Soracan gum product. Taking the crude Soracan gum product alone or taking the crude Soracan gum product with food can reduce liver fat, blood fat, body fat, body weight and cholesterol. The Soracan gum has the advantages of high output and simple and convenient extraction method, can be directly dissolved in cold water, can effectively reduce indexes such as liver fat, blood fat, body fat, body weight, cholesterol and the like, is safe and nontoxic and can be directly eaten.

Description

technical field [0001] The invention relates to the application technology of metabolites of Agrobacterium sp. ZX09 (Chinese name: Solara gum, English name: Salenacan), and application in reducing liver fat, blood fat, body fat, body weight and cholesterol. Background technique [0002] As the economy develops in great strides, the quality of life and way of life of human beings also change. However, the modern lifestyle of "eating well, eating fine, excessive nutrition and less exercise" has also brought diseases to human beings, such as obesity, hyperlipidemia, atherosclerosis, diabetes and so on. According to incomplete statistics, there are currently 130 million fatty liver patients and 50 to 70 million diabetic patients in China. With the rapid economic development, the incidence of these diseases is increasing day by day. There is an urgent need to find efficient and safe control and treatment methods. [0003] At present, many therapeutic drugs have been developed b...

Claims

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Application Information

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IPC IPC(8): A61K31/716A61P1/16A61P3/06A61P3/04A23L1/09A23L33/125
Inventor 张建法周梦怡修爱慧
Owner SICHUAN HETAI SYNLIGHT BIOTECH LTD
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