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Lactobacillus reuteri fermented liquid feed, preparation method and application thereof

A technology of Lactobacillus reuteri and liquid feed, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve the problems of various strains and complicated cultivation process

Active Publication Date: 2013-03-13
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The application number published by the State Intellectual Property Office of China is 200610165284.5, and the invention title is "liquid pig feed fermentation process and equipment". The technology and equipment for fermenting liquid feed have various types of bacteria and complicated cultivation process, and the prepared liquid fermented feed is only used on post-weaning piglets

Method used

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  • Lactobacillus reuteri fermented liquid feed, preparation method and application thereof
  • Lactobacillus reuteri fermented liquid feed, preparation method and application thereof
  • Lactobacillus reuteri fermented liquid feed, preparation method and application thereof

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Effect test

Embodiment 1

[0060] Embodiment 1: Breeding and strain identification of excellent lactic acid bacteria strains

[0061] (1) Isolation and screening of excellent lactic acid bacteria strains in intestinal contents:

[0062] The contents of the cecum of healthy adult pigs were taken aseptically and diluted appropriately with sterile saline. Dip the diluted solution and inoculate it on the MRS plate medium, culture anaerobically at 37°C for 24 hours, pick colonies with different shapes and colors and inoculate them for pure culture, and perform microscopic examination to observe the color and color of the isolated bacteria. Morphological characteristics.

[0063] Pick the purely cultured Gram-positive non-bacillus bacteria on the plate and inoculate them in MRS liquid medium, and culture them anaerobically at 37°C for 24 hours. Add sterilized glycerol to the bacterial culture solution at a ratio of 20%, mix well, store at -20°C, and set aside. They are respectively numbered CL9, L3, WW1, L...

Embodiment 2

[0090] Embodiment 2: the preparation of Lactobacillus reuteri fermented liquid

[0091] (1) Preparation of Lactobacillus reuteri seed liquid:

[0092] Aseptically inoculate the strains preserved on the slant of the test tube into a 500ml Erlenmeyer flask filled with 400ml of MRS medium, and inoculate them anaerobically at a constant temperature of 30°C for 24h.

[0093] (2) Preparation of Lactobacillus reuteri fermented liquid:

[0094] The Lactobacillus reuteri seed solution prepared above was inoculated into a 50L fermenter equipped with 40L fermentation medium according to the inoculum amount of 5%, and incubated anaerobically at a constant temperature of 40°C for 16h.

[0095] The MRS medium of the present invention is: beef extract 1.0g, tryptone 0.5g, yeast extract powder 0.5g, glucose 1.5g, lactose 0.5g, 5% cysteine ​​1.0ml, Tween-800.1ml, magnesium chloride 0.05g, 0.2g ferric ammonium citrate, 0.005g manganese sulfate, 0.5g sodium acetate, 1.4g agar powder, 0.2g anhy...

Embodiment 3

[0097] Embodiment 3, the steps and process parameters of using Lactobacillus reuteri to prepare fermented liquid feed

[0098] (1) Preparation of dry matrix for liquid fermentation of Lactobacillus reuteri: the dry matrix for fermentation of Lactobacillus reuteri according to the present invention is an aniseed part of the complete feed, excluding 4% premix The full-price feed is a conventional basal ration formulated with reference to the nutritional needs of pigs in NRC (1998). Namely: mix 66% of corn, 11% of soybean meal, 14% of bran and 5% of fish meal to obtain a dry matrix for fermentation of Lactobacillus reuteri.

[0099] (2) Dilution of bacterial liquid: Inoculate Lactobacillus reuteri fermented liquid in a certain amount of groundwater or tap water container according to the inoculum amount of 1‰, stir evenly, and stand-by, wherein the liquid loading amount of groundwater or tap water is 50 %.

[0100] (3) The process of Lactobacillus reuteri liquid fermented feed:...

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Abstract

The invention provides a lactobacillus reuteri fermented liquid feed, and a preparation method and application thereof, belonging to the field of microbial fermentation feed and liquid feed. The lactobacillus reuteri fermented liquid feed of the invention is the liquid fermentation feed prepared according to the optimized fermented liquid feed technology with Lactobacillus reuteri (preservation number: CGMCC No.3457) which is subject to directional breeding selection. The lactobacillus reuteri fermented liquid feed comprises not only a dry matrix for fermentation of the lactobacillus reuteri with 108-540 cfu / g of viable bacteria content of the lactobacillus reuteri but also a lactic acid metabolic product with a moisture content of 40-60% and a pH value of 4.5-5.5. The fermented liquid feed also comprises 4% of premix compound which is added after fermentation is completed; and the 4% of premix compound is free from antibiotics. The fermented liquid feed of the invention features in simple technique, easy operation by free-range keepers and can carry out good fermentation in extensive pig farm environment. In addition, the fermented liquid feed enjoys remarkable effect in stimulating growth of weaned pigs, growing pigs and growing and fattening pigs. The feed can improve efficiency of feed utilization, promote growth of animals, improve and maintain balance of intestinal flora, improve immunity and premonition of animals, improve animal body type and product quality, noticeably reduce feed cost and increase economic benefits.

Description

technical field [0001] The invention relates to a Lactobacillus reuteri fermented liquid feed and a preparation method and application thereof, belonging to the field of microbial fermented feed and liquid feed. Background technique [0002] Fermented Liquid Feed (Fermented Liquid Feed, abbreviated as FLF), as a new type of feed, was first used in the Netherlands in the late 1980s. After that, Denmark, France, Sweden, Spain, Switzerland and other countries also joined in the use. In recent years, fermented liquid feed has been widely used in Europe and has become a research hotspot. It can improve the digestibility of feed and improve the production performance of pigs, especially in preventing diarrhea of ​​weaned piglets, increasing feed intake and daily weight gain. But there are still some key technical issues to be solved: the development of suitable strains for fermented liquid feed, the fermentation process of fermented liquid feed, especially the quality of strains ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00C12Q1/04C12R1/225A23K10/12A23K10/30A23K50/30
Inventor 王金全蔡辉益王春阳陈鲜鑫孙占敏张广民王旭莉李红霞胡丽博
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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