Novel fish odinagogue

A technology for oxytocin and fish, applied in the field of new type of oxytocin for fish, which can solve problems such as failure to start, residual eggs, reproductive defects, etc.

Inactive Publication Date: 2011-01-19
SUN YAT SEN UNIV
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] However, when the gonads of many fish are mature, due to unsuitable environmental conditions, the fish cannot ovulate normally, and the eggs remain in the body and slowly degenerate, which brings a lot of pressure to the seedling demand for fish farming. Therefore, artificial oxytocin for fish is an important way to meet the

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  • Novel fish odinagogue

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Embodiment Construction

[0011] By cloning the gene kiss1 of goldfish kisspeptin, and then analyzing the cloned sequence according to information biology methods, it is speculated that the decapeptide (kiss1-10) of the goldfish kisspeptin gene exerts biological activity: Tyr Asn Lys Asn Ser Phe Gly Lys Arg Tyr. These ten amino acid peptides were synthesized by artificial chemistry. The specific process can be found in Journal of Endocrinology (2009) 201, 407-418. The polypeptide of the above amino acid sequence is used as an exogenous drug for artificially inducing ovulation in fish to induce labor in fish.

[0012] Twenty goldfish with mature ovaries were selected and divided into control group and treatment group, with 10 fish in each group. The control group was injected with physiological saline, and the treatment group was injected with a polypeptide (kiss1-10) consisting of the amino acid sequence Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr. Dissolve the white powdered kiss1-10 in 0.7% normal sali...

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Abstract

The invention discloses a novel fish odinagogue, which is a polypeptide having an amino acid sequence of Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr or a polypeptide having an amino acid sequence which has an over 60-percent homology with the amino acid sequence of Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr. When use, the polypeptide is dissolved in normal saline for fish according to a ratio of 1 milligram peptide per milliliter of normal saline; the injection amount is 0.5 to 1mu g per gram of fish; and the solution is injected into the fish at a position near the base part of the pectoral fin or the muscle of the dorsal fin. The novel fish odinagogue can induce the ovulation of goldfish, crucian and loach, and is novel fish odinagogue with an important production prospect.

Description

technical field [0001] The invention relates to a novel fish oxytocic agent for artificially inducing fish ovulation in aquaculture. Background technique [0002] The final maturation and ovulation of fish gonads are controlled by hormones secreted by the hypothalamus-pituitary-gonad axis. In addition, environmental factors also have a great influence on the reproductive activities of fish. The sensory organs transmit the stimuli of the external environment (such as temperature, light, rainfall, etc.) to the brain, causing the hypothalamus to secrete gonadotropin-releasing hormone (GnRH), which stimulates the pituitary gland to secrete gonadotropin (GtH) to act on the gonads and promote gonad secretion Sex steroid hormones to promote the maturation of gonads and release sperm and eggs. [0003] However, when the gonads of many fish are mature, due to unsuitable environmental conditions, the fish cannot ovulate normally, and the eggs remain in the body and slowly degenerate,...

Claims

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Application Information

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IPC IPC(8): A61K38/08A61P15/08
Inventor 刘晓春李水生张勇刘云林浩然
Owner SUN YAT SEN UNIV
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