Novel fish odinagogue
A technology for oxytocin and fish, applied in the field of new type of oxytocin for fish, which can solve problems such as failure to start, residual eggs, reproductive defects, etc.
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[0011] By cloning the gene kiss1 of goldfish kisspeptin, and then analyzing the cloned sequence according to information biology methods, it is speculated that the decapeptide (kiss1-10) of the goldfish kisspeptin gene exerts biological activity: Tyr Asn Lys Asn Ser Phe Gly Lys Arg Tyr. These ten amino acid peptides were synthesized by artificial chemistry. The specific process can be found in Journal of Endocrinology (2009) 201, 407-418. The polypeptide of the above amino acid sequence is used as an exogenous drug for artificially inducing ovulation in fish to induce labor in fish.
[0012] Twenty goldfish with mature ovaries were selected and divided into control group and treatment group, with 10 fish in each group. The control group was injected with physiological saline, and the treatment group was injected with a polypeptide (kiss1-10) consisting of the amino acid sequence Tyr-Asn-Lys-Asn-Ser-Phe-Gly-Lys-Arg-Tyr. Dissolve the white powdered kiss1-10 in 0.7% normal sali...
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