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Method for reducing branch quantity of plant and improving chlorophyll and anthocyanin contents of plant

An anthocyanin and chlorophyll technology, which is applied in the fields of botanical equipment and methods, angiosperms/flowering plants, plant products, etc., can solve the problems of slow growth of transgenic plants and no branching phenotype, and achieves a simple and easy construction method. row, branch count reduction, effect of branch count reduction

Active Publication Date: 2012-06-27
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This patent application is only Figure 5 The fourth panel of A shows that the antisense-transformed transgenic plants developed significantly slower, but did not show branching phenotypes

Method used

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  • Method for reducing branch quantity of plant and improving chlorophyll and anthocyanin contents of plant
  • Method for reducing branch quantity of plant and improving chlorophyll and anthocyanin contents of plant
  • Method for reducing branch quantity of plant and improving chlorophyll and anthocyanin contents of plant

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Effect test

Embodiment 1

[0056] Construction of transgenic plants in Arabidopsis thaliana overexpressing the miR171 gene to reduce the number of branches in plants

[0057] Taking Arabidopsis miR171c as an example, the construction process of transgenic plants overexpressing miR171 is illustrated. The PCR-amplified Arabidopsis thaliana miR171c gene was cloned into the BamHI and XbaI sites of the pBS vector, and after sequencing, the miR171c fragments were excised with BamHI and XbaI, and inserted into pKY71 respectively (Schardl et al., 1987, Gene, 61 : 1-11) vector or pHB (Mao et al., PNAS, 2005, 102: 12270-12275) behind the 35S promoter of the vector to obtain a plant expression vector for overexpressing miR171, and transform the Agrobacterium GV3101 strain with the obtained plasmid. Using the obtained bacterial strains, this vector was transformed into plants by flower dipping method (Clough and Bent, 1998, Plant J, 16: 735-743) to obtain 35Spro-MIR171c transgenic plants overexpressing the miR171c ...

Embodiment 2

[0059] Construction of transgenic plants overexpressing the rice miR171 gene in Arabidopsis to reduce the number of branches in plants

[0060] Taking the rice miR171a gene (Os-miR171a, Sunkar et al., 2005, Plant Cell, 17: 1397-1411. Gene number: Os06G13106) as an example, the construction process of transgenic plants overexpressing Os-miR171a is illustrated. The fragment of Os-miR171a amplified by PCR was cloned into the BamHI and XbaI sites of the pBS vector, and after sequencing, the fragment of Os-miR171a was excised with BamHI and XbaI, and inserted into the back of the 35S promoter of the pKY71 vector or PHB vector respectively to obtain A plant expression vector that overexpresses Os-miR171a ( figure 2 A), using the obtained plasmid to transform the Agrobacterium GV3101 strain. The obtained bacterial strain is used to transform the vector into plants by flower dipping method to obtain transgenic plants. The expression level of Os-miR171a was detected by the northern ...

Embodiment 3

[0062] Construction of scl6-II scl6-III double mutant and scl6-II scl6-III scl6-IV triple mutant in Arabidopsis to reduce the number of branches in plants

[0063] Take Arabidopsis as an example to illustrate the acquisition process of SCL6-II, SCL6-III and SCL6-IV gene function loss or reduction mutants. Create mutant library of dicotyledonous plants by T-DNA insertion method. Using the seedlings of the M1 generation, the SCL6-II, SCL6-III and SCL6-IV sites of the above mutants were amplified by the PGR method, and the mutation sites were determined by sequencing. After the determined single mutants of scl6-II, scl6-III and scl6-IV are obtained, the scl6-II can be crossed with the scl6-III mutant to obtain F1 generation seeds. The F2 generation seedlings were screened by PCR, and the scl6-II scl6-III double mutant plants homozygous for both SCL6-II and SCL6-III mutation sites were selected, and then crossed with the scl6-IV mutant. After the F1 generation is selfed, the F2 ...

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Abstract

The invention discloses a method for reducing branch quantity of a plant and improving chlorophyll and anthocyanin contents of the plant by using an miR171 gene and target genes SCL6 thereof, which relates to the technical field of agricultural science. The invention comprises (1) a method for reducing the branch of a plant, improving the chlorophyll and anthocyanin contents of the plant and enhancing anti-aging and drought resistances of the plant through the function over-expression of the miR171 gene and the function loss or reduction of SCL6-II, SCL6-III and SCL6-IV genes; and (2) a method for reducing the branch quantity of the plant, improving the chlorophyll and anthocyanin contents of the plant and enhancing the anti-aging and drought resistances of the plant through the function over-expression of the miR171 gene and the function loss or reduction of the SCL6-II, SCL6-III and SCL6-IV genes. The invention has application of the miR171 gene, the SCL6-II gene, the SCL6-III gene and the SCL-IV 6 gene in reducing the branch quantity of the plant and improving the chlorophyll and anthocyanin contents of the plant, the function of the miR171 gene in the anti-aging and drought resistances and the application thereof in enhancing the anti-aging and drought resistances. The obtained effect of the invention is obvious.

Description

technical field [0001] The present invention relates to a method for regulating the number of plant branches and the content of chlorophyll and anthocyanin in the field of agricultural science and technology. Specifically, it relates to using miR171 and its target gene SCL6 to reduce the number of plant branches and increase the amount of chlorophyll and anthocyanins. Methods for anthocyanin content. Background technique [0002] Throughout the evolution of plants, different plants have different requirements for ideal branching. If the branching of various plants can be regulated by technical means, it will play a very important role. Plant branching is initiated by meristems in leaf axils, and this process is regulated by a complex network. Secondary meristems give rise to a series of axillary meristems of equal developmental potential that either choose to develop as collaterals or become dormant. This process is tightly regulated by developmental stage, environmental ...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
Inventor 杨洪全王龙
Owner SHANGHAI JIAO TONG UNIV
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