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Separating identification of new compound furostanol saponin in smilacina japonica and antitumor application thereof

A technology of compound and furosemide, applied in the field of natural medicinal chemistry, can solve problems such as restricting the development and utilization of deer medicine resources

Inactive Publication Date: 2012-05-23
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the relevant medicinal ingredients and modern pharmacological research are almost blank, which restricts the development and utilization of deer medicinal resources.

Method used

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  • Separating identification of new compound furostanol saponin in smilacina japonica and antitumor application thereof
  • Separating identification of new compound furostanol saponin in smilacina japonica and antitumor application thereof
  • Separating identification of new compound furostanol saponin in smilacina japonica and antitumor application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Extraction, separation and identification of new compounds

[0021] 1. Experimental materials and methods

[0022] 1.1 Sample

[0023] The deer medicine is collected from Tumenling area, Jiutai City, Jilin Province. It is identified by Professor Yang Limin of the College of Chinese Materia Medica, Jilin Agricultural University. The rhizomes are washed, dried naturally in the shade, crushed mechanically, and used for later use.

[0024] 1.2 Instruments and reagents

[0025] Instrument: DL-1000E Intelligent Ultrasonic Cleaner (Shanghai Zhixin Instrument Co., Ltd.)

[0026] RE52-99 rotary evaporator (Shanghai Yarong Biochemical Instrument Factory)

[0027] Agilent 1100 HPLC (Agilent Technologies, Inc., USA)

[0028] X-4 digital microscopic melting point tester (temperature uncorrected, Beijing Tektronix Instrument Co., Ltd.)

[0029] FTIR-8400S Fourier transform infrared spectrometer (KBr pellets, Shimadzu, Japan)

[0030] UV-1700 ultraviolet spe...

Embodiment 2

[0049] Antitumor activity detection of embodiment two new compounds

[0050] 1. Experimental materials and methods

[0051] 1.1 Instruments and reagents

[0052] Instrument: 96-well cell culture plate (Costa Company)

[0053] Incubator (NUAIRΞUS AUTOFLOW CO 2 Water-Jacketed Incubator)

[0054] Ultra-clean workbench (Shanghai Boxun Industrial Co., Ltd. Medical Equipment Factory)

[0055] Nikon ecl ipses TS100

[0056] TS-2 decolorization shaker (Haimen Qilinbeier Instrument Manufacturing Co., Ltd.)

[0057] DG5032 ELISA (Nanjing Huadong Electronics Group Medical Equipment Co., Ltd.)

[0058] Reagent: newborn bovine serum (Hangzhou Sijiqing Bioengineering Materials Co., Ltd.)

[0059] RPMI-1640 (Gibco, USA)

[0060] Trypsin, thiazolium blue (MTT), Trypan Blue (Amresco)

[0061] 5-fluorouracil (5-Fu) and cisplatin (DDP) (purchased from China-Japan Friendship Hospital of Jilin University, Changchun)

[0062] Dimethyl sulfoxide (DMSO) (Tianjin Beilian Fine Chemicals Devel...

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Abstract

The invention relates to separating identification of new compound furostanol saponin in smilacina japonica and antitumor application thereof. In the invention, a new furostanol saponin compound is obtained by design and separating smilacina japonica and has a name of 26-O-beta-D-glucopyranosyl-(25R)-furost-5-en-3beta,12,17alpha,22xi,26-pentol-12-O-acetyle-3-O-alpha-L-rhamnopyranosyl-(1->2)-beta-D-glucopyranoside. The invention carries out antitumor activity test on the new compound by adopting an MTT (Microwave Theory and Technique) method; and indicated by test results, the compound has activity for inhibiting human lung adenocarcinoma SPC-A-1 cell growth, and an IC50 value is 7.67 mug.mL<-1>.

Description

technical field [0001] The invention relates to the field of natural medicinal chemistry, in particular to the isolation and identification of a novel furostanol saponin compound and its activity against human lung adenocarcinoma SPC-A-1 cells. Background technique [0002] Deer medicine is the dried rhizome and root of Smilacina japonica A.Gray, a perennial herb of the genus Smilacina japonica A.Gray in the Liliaceae family. It is a commonly used traditional Chinese medicine. There are about 25 species of deer medicine plants in the world, and 14 species and 2 varieties in my country. Except Xinjiang, Qinghai, Ningxia and Inner Mongolia, they are distributed all over the country, and the reserves of wild resources are very rich. The plants of the genus deer are of medicinal and edible value. Except for the rhizomes and roots, they can be used as medicine. The young stems and leaves on the ground are popular mountain vegetables. Among them, the species of deer are the most w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00A61K31/7048A61P35/00A61P11/00
Inventor 赵淑杰杨利民韩梅战杨韩忠明
Owner JILIN AGRICULTURAL UNIV
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