Inverted liquid-phase acoustic surface wave detection device and array thereof

Abstract

The invention discloses an inverted liquid-phase acoustic surface wave detection device, which comprises a substrate and a reaction tank, wherein the substrate is provided with at least one pair of interdigital transducers, and the input interdigital transducer, the output interdigital transducer and lead areas thereof are provided with waterproof isolation layers; and the substrate is verticallyinserted into the reaction tank so that the detection area is soaked into the reaction tank. The reaction tank with constant size for bearing reaction liquid is adopted in the device so as to ensure consistency of the injected liquid amount in repeated experiments and solve the problem that the consistency of the liquid dropping amount in the repeated experiments is difficult to ensure in liquid dropping detection of the traditional acoustic surface wave sensor in the detection area; the substrate is vertically inserted into the reaction tank to form a closed reaction area, so the liquid sample cannot be volatilized and the defect that the liquid sample is easy to volatilize in the liquid dropping detection of the acoustic surface wave sensor is overcome; compared with the conventional acoustic surface wave detection device for detection through microfluidic inlet liquid, the standing closed reaction structure avoids liquid flowing interference; and the inverted liquid-phase acoustic surface wave detection device is arranged in an array form to realize simultaneous detection of multiple detected liquid samples.

Description

technical field [0001] The invention relates to the field of liquid-phase surface acoustic wave sensors, in particular to an inverted insertion type liquid-phase surface acoustic wave detection device and an array thereof. Background technique [0002] The surface acoustic wave sensor (SAW sensor) includes an input interdigital transducer (IDT) and an output interdigital transducer set on the substrate. The input electrical signal is converted by the input interdigital transducer into an Polarized transverse wave, the polarized transverse wave is transmitted along the substrate to the output interdigital transducer, the polarized transverse wave is very sensitive to the specific biological response on the surface of the sensor during the transmission process, and the propagation speed, amplitude and phase of the sound wave pass through the sensitive area. Change accordingly. The changed sound wave signal is then converted into an electrical signal through an output interdig...

AI Technical Summary

Problems solved by technology

[0003] In the biomolecular reaction detection of the prior art, the detection area of ​​the surface acoustic wave sensor adopts 1) microfluidic control to enter the liquid, and 2) directly drops the detection liquid sample in the detection area to detect the biomolecular reaction. The advantage is that it is convenient for sample injection and washing, and the detection is continuous, but because the liquid flow disturbs the sensitive sensor signal, the liquid flow rate is required to be very low, but this is difficult to control and cannot be stabilized

Moreover, liquid flow will affect the kinetic characteristics of the binding reaction between biomolecules, which may destroy the authenticity of the detection results.

Although the liquid sample is stable in the second sample injection method, the amount of liquid dripping needs to be the same every time, and the area covered by the sensor surface must also be the same to ensure the repeatability of the detection, thus increasing the difficulty of sample addition and washing the sample. Inconvenient; since the liquid sample is not sealed, the dripped sample is easy to volatilize and dry under a certain temperature control system when the temperature is high, so that the detection cannot be carried out

Images