Cell freezing solution without complicated procedures for freezing

Abstract

The invention provides a solution for non-programmed cell cryopreservation, which comprises 1.0-28 w / v % of cell membrane protectant, 1.0-18 w / v % of permeable intracellular protectant, 3.0-28 w / v % of cell sedimentation stabilizer, and the balance of solvent. The cell cryopreservation solution provided by the invention is good for protecting cells. After the cryopreservation solution is added to cells, the cells can be cryopreserved in a refrigerator of −80 DEG C. directly without complicated programmed cryopreservation, thus, the time for cell cryopreservation is shortened greatly and the efficiency of cryopreservation is improved. Therefore, the cryopreservation solution is suitable for cryopreserving a large number of cells. The recovery rate of the cells cryopreserved is high, the growth and differentiation of the recovered cells is normal. The components of the cryopreservation solution are stable, thus the cryopreservation solution can be preserved with good property for a long time.

Description

technical field [0001] The invention relates to a cell cryopreservation solution, in particular to a non-programmed cell cryopreservation solution. Background technique [0002] Cells, especially high-value cells such as stem cells, have potential medical value and other values, and cell preservation technology is the basis for realizing these values. [0003] Commonly used cell preservation methods include culture preservation and cryopreservation. Cultivation and preservation are time-consuming and labor-intensive, and the procedures are cumbersome. During the cultivation process, especially during long-term subculture, cells are prone to mutation, cell characteristics are lost, and preservation value is lost. Therefore, culture preservation is generally applied to cells that are easy to culture and not easy to mutate, such as some tumor cells. [0004] Cell cryopreservation is to store cells in a low temperature environment, so that cells can temporarily escape from the...

AI Technical Summary

Problems solved by technology

This kind of freezing solution is generally a ready-to-use freezing solution, which needs to be prepared and used immediately, with large batch differences, short storage period, and unstable storage effect

[0006] In addition, the cryopreservation procedure of the existing cryopreservation solution is complex, requiring the use of expensive special cryopreservation equipment for programmed cooling. The entire cryopreservation process takes up to 3 to 4 hours to ensure the recovery rate of cryopreserved cells

This cryopreservation operation is complicated, the processing volume is small, and the cryopreservation cost is high, which cannot meet the needs of large-scale cell rapid cryopreservation

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