Cell freezing solution without complicated procedures for freezing
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A technology for cryopreservation and cells, applied in the field of cell cryopreservation, can solve the problems of inability to meet the needs of rapid cryopreservation of large-scale cells, unstable preservation effect, complicated cryopreservation procedures, etc. The effect of normal growth and differentiation, improving the efficiency of cryopreservation
Active Publication Date: 2011-03-09
CYAGEN BIOSCI INC
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This kind of freezing solution is generally a ready-to-use freezing solution, which needs to be prepared and used immediately, with large batch differences, short storage period, and unstable storage effect
[0006] In addition, the cryopreservation procedure of the existing cryopreservation solution is complex, requiring the use of expensive special cryopreservation equipment for programmed cooling. The entire cryopreservation process takes up to 3 to 4 hours to ensure the recovery rate of cryopreserved cells
This cryopreservation operation is complicated, the processing volume is small, and the cryopreservation cost is high, which cannot meet the needs of large-scale cell rapid cryopreservation
Method used
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Embodiment 1
[0039] The composition of the cell freezing solution is as follows:
[0057] Permeable intramembrane protectant 1%, consisting of propylene glycol,
[0058] Cell Sediment Stabilizer 9%, consisting of Hetastarch,
[0059] Antioxidant 0.1%, consisting of glutathione,
[0060] Cell Nutrition 0.2%, consisting of Sodium Pyruvate,
[0061] Newborn bovine serum, balance.
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Abstract
The invention discloses a cell freezing solution without complicated procedures for freezing, which comprises 1.0-28w / v% of cell membrane protective agent, 1.0-18w / v% of permeable cell membrane inner protective agent, 3.0-28% of cell settlement stabilizer and balance of solvent. The cell freezing solution of the invention has good protective effect on cells, after the freezing solution is added, the cells can be directly put into a refrigerator of -80DEG C for freezing, and complicated procedures are not needed for freezing, thereby greatly shortening the freezing time of the cells, improvingthe freezing efficiency and being suitable for freezing a large quantity of cells. The recovery rate of the frozen cells is high, the growth and differentiation of the recovered cells are normal, thecomponents of the cell freezing solution have stability and a long quality guarantee period, and the cell freezing solution does not need to be prepared or diluted before use and has good batch stability.
Description
technical field [0001] The invention relates to a cell cryopreservation solution, in particular to a non-programmed cell cryopreservation solution. Background technique [0002] Cells, especially high-value cells such as stem cells, have potential medical value and other values, and cell preservation technology is the basis for realizing these values. [0003] Commonly used cell preservation methods include culture preservation and cryopreservation. Cultivation and preservation are time-consuming and labor-intensive, and the procedures are cumbersome. During the cultivation process, especially during long-term subculture, cells are prone to mutation, cell characteristics are lost, and preservation value is lost. Therefore, culture preservation is generally applied to cells that are easy to culture and not easy to mutate, such as some tumor cells. [0004] Cell cryopreservation is to store cells in a low temperature environment, so that cells can temporarily escape from the...
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