Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell freezing solution without complicated procedures for freezing

A technology for cryopreservation and cells, applied in the field of cell cryopreservation, can solve the problems of inability to meet the needs of rapid cryopreservation of large-scale cells, unstable preservation effect, complicated cryopreservation procedures, etc. The effect of normal growth and differentiation, improving the efficiency of cryopreservation

Active Publication Date: 2011-03-09
CYAGEN BIOSCI INC
View PDF2 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of freezing solution is generally a ready-to-use freezing solution, which needs to be prepared and used immediately, with large batch differences, short storage period, and unstable storage effect
[0006] In addition, the cryopreservation procedure of the existing cryopreservation solution is complex, requiring the use of expensive special cryopreservation equipment for programmed cooling. The entire cryopreservation process takes up to 3 to 4 hours to ensure the recovery rate of cryopreserved cells
This cryopreservation operation is complicated, the processing volume is small, and the cryopreservation cost is high, which cannot meet the needs of large-scale cell rapid cryopreservation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell freezing solution without complicated procedures for freezing
  • Cell freezing solution without complicated procedures for freezing
  • Cell freezing solution without complicated procedures for freezing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The composition of the cell freezing solution is as follows:

[0040] Cell membrane protective agent 1%, composed of 0.4% raffinose, 0.6% middle molecular anhydrous glycoside-70,

[0041] Permeable intramembrane protectant 5%, consisting of DMSO,

[0042] Cell Sediment Stabilizer 15%, consisting of Methylcellulose 500CP,

[0043] Antioxidant 0.2%, composed of 0.03% vitamin C, 0.17% glutathione,

[0044] Cell nutrition 0.3%, composed of 0.2% glutamine, 0.1% sodium pyruvate,

[0045] Newborn bovine serum, balance.

Embodiment 2

[0047] The composition of the cell freezing solution is as follows:

[0048] Cell membrane protector 7%, composed of trehalose,

[0049] Permeable cell membrane inner protector 9%, composed of 6% propylene glycol, 3% glycerol,

[0050] Cell sedimentation stabilizer 28%, composed of 20% methylcellulose 400CP, 8% dextrin,

[0051] Antioxidants 0.4%, consisting of Vitamin C,

[0052] Cell Nutrition 1.0%, composed of 0.7% glutamine, 0.3% sodium pyruvate,

[0053] Newborn bovine serum, balance.

Embodiment 3

[0055] The composition of the cell freezing solution is as follows:

[0056] Cell membrane protective agent 28%, composed of 10% sucrose, 14% panose, 4% low-molecular dextran-40,

[0057] Permeable intramembrane protectant 1%, consisting of propylene glycol,

[0058] Cell Sediment Stabilizer 9%, consisting of Hetastarch,

[0059] Antioxidant 0.1%, consisting of glutathione,

[0060] Cell Nutrition 0.2%, consisting of Sodium Pyruvate,

[0061] Newborn bovine serum, balance.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cell freezing solution without complicated procedures for freezing, which comprises 1.0-28w / v% of cell membrane protective agent, 1.0-18w / v% of permeable cell membrane inner protective agent, 3.0-28% of cell settlement stabilizer and balance of solvent. The cell freezing solution of the invention has good protective effect on cells, after the freezing solution is added, the cells can be directly put into a refrigerator of -80DEG C for freezing, and complicated procedures are not needed for freezing, thereby greatly shortening the freezing time of the cells, improvingthe freezing efficiency and being suitable for freezing a large quantity of cells. The recovery rate of the frozen cells is high, the growth and differentiation of the recovered cells are normal, thecomponents of the cell freezing solution have stability and a long quality guarantee period, and the cell freezing solution does not need to be prepared or diluted before use and has good batch stability.

Description

technical field [0001] The invention relates to a cell cryopreservation solution, in particular to a non-programmed cell cryopreservation solution. Background technique [0002] Cells, especially high-value cells such as stem cells, have potential medical value and other values, and cell preservation technology is the basis for realizing these values. [0003] Commonly used cell preservation methods include culture preservation and cryopreservation. Cultivation and preservation are time-consuming and labor-intensive, and the procedures are cumbersome. During the cultivation process, especially during long-term subculture, cells are prone to mutation, cell characteristics are lost, and preservation value is lost. Therefore, culture preservation is generally applied to cells that are easy to culture and not easy to mutate, such as some tumor cells. [0004] Cell cryopreservation is to store cells in a low temperature environment, so that cells can temporarily escape from the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226A01N2300/00
Inventor 郑敦武莫筱萦魏宝丽陈嘉慧罗晴燕
Owner CYAGEN BIOSCI INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com