Unlock instant, AI-driven research and patent intelligence for your innovation.
Method for preparing lactobionic acid by microbial transformation
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of microbial transformation and lactobionic acid, applied in the field of food biology, can solve the problems of complicated separation and purification steps, etc.
Inactive Publication Date: 2011-03-23
JIANGNAN UNIV
View PDF1 Cites 13 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
There are many unfavorable factors in the chemical catalytic synthesis method, such as the production of various by-products and chemical pollutants, and the formation of many by-products complicates the separation and purification steps
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0025] Embodiment 1. The influence of fermentation time on lactobionic acid output
[0026] According to the fermentation conditions described in the instructions, the substrate in the fermentation medium is 3% lactose, by Pseudomonas flurosescence The detection of lactobionic acid produced by SK17.001 at different fermentation times found that the content of lactobionic acid was the highest at 60 hours of fermentation, and the effect of fermentation time on the production of lactobionic acid is shown in Table 1.
[0027] Table 1. Effect of fermentation time under 3% lactose concentration on conversion to lactobionic acid
[0031] 3% Lactose, 0.5% CaCO 3 , initial pH6.2, temperature 30°C, 220rpm, Pseudomonasfluorescence When SK 17.001 was cultured for 60 hours, the conversion rate of lactose into lactobionic acid could reach 91%.
[0032] 10% Lactose, 1.5% CaCO 3 , initial pH6.2, temperature 30℃, 220rpm, Pseudomonasfluorescence When SK 17.001 was cultivated for 120 hours, the conversion rate of lactose into lactobionic acid could reach 80%.
[0033] 20% Lactose, 3% CaCO 3 , initial pH 6.2, temperature 30°C, 220rpm, Pseudomonas fluorescence When SK 17.001 was cultured for 120 hours, the conversion rate of lactose into lactobionic acid could reach 62.5%.
[0034] Add different concentrations of substrates in the medium, use the above-mentioned preferred medium for Pseudomonas flurosescence SK 17.001 was transformed and detected after the transformation time, and it was found that with the increase of lac...
Embodiment 3
[0037] Example 3 The effect of adding different carbon sources on the conversion rate in addition to the substrate
[0038] 20% Lactose, 2% Fructose, 3% CaCO 3 , initial pH 6.2, temperature 30°C, 220rpm, Pseudomonas fluorescence When SK 17.001 was cultured for 120 hours, the conversion rate of lactose into lactobionic acid could reach 90%.
[0039] 20% Lactose, 2% Glucose, 3% CaCO 3 , initial pH 6.2, temperature 30°C, 220rpm, Pseudomonas fluorescence When SK 17.001 was cultivated for 120 hours, the conversion rate of lactose into lactobionic acid could reach 84%.
[0040] 20% Lactose, 2% Glycerin, 3% CaCO 3 , initial pH 6.2, temperature 30°C, 220rpm, Pseudomonas fluorescence When SK 17.001 was cultured for 120 hours, the conversion rate of lactose into lactobionic acid could reach 70%.
[0041] 20% Lactose, 0.2% Fructose, 3% CaCO 3 , initial pH 6.2, temperature 30°C, 220rpm, Pseudomonas fluorescence When SK 17.001 was cultivated for 120 hours, the conversion rate o...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for preparing lactobionic acid by microbial transformation, and belongs to the technical field of food biology. The invention provides a strain for preparing the lactobionic acid by microbial transformation, which is classified and named Pseudomonasfluorescens SK17.001 and has been collected in China Center for Type Culture Collection with the collection number CCTCC NO:M2010216. The Pseudomonasfluorescens SK17.001 microbe is used as the strain, lactin is taken as a transformation substrate and is added with a nitrogen source and inorganic salt to form a fermentation medium, and the lactobionic acid is prepared by the microbial transformation method. In the fermentation process, an alkaline substance CaCO3 is added, so the pH is kept constant, and the conversion rate of the lactin is improved. Under preferable conditions, the conversion rate is over 90 percent. The lactobionic acid product prepared by the method is safe and reliable, can be used as a pharmaceutical intermediate and food additive, and has physiological functions of enhancing the absorption of insoluble mineral substances and the like.
Description
technical field [0001] The invention relates to a method for preparing lactobionic acid, in particular to a method for preparing lactobionic acid through microbial conversion, and belongs to the field of food biotechnology. Background technique [0002] Lactobionic acid is the third-generation fruit acid, and it is the most advanced fruit acid that integrates various functions such as anti-aging, moisturizing, anti-oxidation and promoting body renewal. Since it exists in the body itself, there is no irritation problem, and it has excellent antioxidant capacity. It is used in organ transplantation to prevent organs from being damaged by free radicals; lactobionic acid can also prevent damage to cell membranes due to oxidation. , has excellent water retention and is an important component in cosmetics. Lactobionic acid is an important component in organ transplantation buffer, and the product after metalchelation can reduce the damage to the tissue due to the hydroxyl group ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More