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Loop-mediated isothermal amplification reaction primer for detecting B substock avian leukosis

A ring-mediated isothermal and amplification reaction technology is applied in the detection field of B subgroup avian leukemia, which can solve the problems of few typing detection methods and the inability of antibody detection kits to distinguish A subgroup, etc., so as to achieve good applicability and reduce The possibility of contamination, the effect of high accuracy

Inactive Publication Date: 2011-03-23
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

However, there are relatively few typing detection methods for subgroup B at present, and commercial antibody detection kits cannot be distinguished from subgroup A.

Method used

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  • Loop-mediated isothermal amplification reaction primer for detecting B substock avian leukosis
  • Loop-mediated isothermal amplification reaction primer for detecting B substock avian leukosis
  • Loop-mediated isothermal amplification reaction primer for detecting B substock avian leukosis

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Embodiment 1

[0016] 1 Materials and methods

[0017] 1.1 virus

[0018] J subgroup avian leukemia HPRS-103 strain, A subgroup avian leukemia RAV-1 strain, B subgroup avian leukemia RAV-2 strain, C subgroup avian leukemia RAV-49 strain, E subgroup avian leukemia RAV-0 strain, Chicken infectious bursal disease supervirulent Gx strain, chicken infectious anemia virus (CAV) M9905 strain, and avian reticuloendotheliosis virus HLJR0901 strain are preserved by the research group of poultry immunosuppressive diseases of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (The above-mentioned strains can be purchased from the China Veterinary Drug Control Institute). Avian influenza virus (AIV), Newcastle disease virus (NDV), chicken infectious bronchitis virus (IBV), and chicken Marek's disease virus (MDV) were donated by the corresponding research groups of Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (the above strains can also be pu...

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Abstract

The invention discloses a loop-mediated isothermal amplification reaction primer for detecting B substock avian leukosis, which is composed of a pair of outer primers, a pair of inner primers and a pair of loop primers, wherein the outer primers are composed of a primer with SEQ ID NO.1 and a primer with SEQ ID NO.2; the inner primers are composed of a primer with SEQ NO.3 and a primer with SEQ NO.4; and the loop primers are composed of a primer with SEQ ID NO.5 and a primer with SEQ ID NO.6. In the loop-mediated isothermal amplification reaction primer for detecting the B substock avian leukosis provided by the invention, a B substock LAMP primer is designed by selecting an avian leukoviruses gp85 section, and according to the designed primer, a method for detecting ALV-B type LAMP is built; the detection method can not be used for carrying out cross reaction with other main substocks of the avian leukosis and can not be used for carrying out heterogenetic reaction with other common poultry communicable diseases at the same time; and the detection method is convenient and accurate, the local applicability is good, the accuracy is high, the specificity is strong and the sensitivity is high.

Description

technical field [0001] The invention relates to a loop-mediated isothermal amplification reaction primer, in particular to a loop-mediated isothermal amplification reaction primer for detecting B subgroup avian leukemia, and belongs to the detection field of B subgroup avian leukemia. Background technique [0002] Subgroup B avian leukemia is caused by subgroup B avian leukemia virus (avian leukemia virus-B, ALV-B), mainly causing lymphocytic leukemia and sarcoma. In the 1970s, Graf et al. and Kawai et al. reported subgroup B avian leukemia respectively. After 1987, large international breeder companies have basically achieved ALV purification, and there have been few cases of subgroup B leukemia reported since then. But after 2000, reports of natural infection of subgroup B appeared again. Spencer et al detected both ALV-A and ALV-B from commercial chicken flocks. Gingerich et al. found recombinant viruses of J and B subgroups in Bailaihang chickens. The LTR of the virus...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
Inventor 王笑梅王永强高宏雷高玉龙秦立廷祁小乐
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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