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Microrna-based methods and compositions for the diagnosis, prognosis and treatment of gastric cancer

A composition and gastric cancer technology, applied in biochemical equipment and methods, DNA/RNA fragments, drug combinations, etc., can solve problems such as difficult diagnosis and treatment

Inactive Publication Date: 2011-04-06
THE OHIO STATE UNIV RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Despite considerable research into therapies to treat such diseases, they remain difficult to diagnose and treat effectively, and the observed mortality rates in patients suggest a need for improved diagnosis, treatment and prevention of the diseases

Method used

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  • Microrna-based methods and compositions for the diagnosis, prognosis and treatment of gastric cancer
  • Microrna-based methods and compositions for the diagnosis, prognosis and treatment of gastric cancer
  • Microrna-based methods and compositions for the diagnosis, prognosis and treatment of gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0117] Dysregulation of miRNA expression in human gastric cancer

[0118] The majority of gastric adenocarcinomas occur in the setting of a chronic inflammatory background commonly associated with Helicobacter Pylori (HP) infection (Uemura et al., 2001). However, the molecular mechanisms responsible for the oncogenicity of HP remain unclear, although Th1 immune responses appear to be critical in the development of precancerous lesions such as gastric atrophy and intestinal metaplasia (Houghton et al., 2002; Fox et al., 2000).

[0119] In the search for miRNAs that may be involved in gastric tumorigenesis, we analyzed 20 intestinal-type gastric primary tumors (each paired with adjacent non-neoplastic gastric tissue from the same patient) and 6 gastric primary tumors using custom-made miRNA microarrays. Overall miRNA expression in gastric cancer cell lines. To identify specific alterations associated with inflammation and / or premalignant lesions, we first compared non-neoplasti...

Embodiment II

[0190] Tissue samples:

[0191] Primary gastric tumor samples were obtained from the Department of Histopathology (Sant'Andrea Hospital, University of Rome "La Sapienza", Italy). All samples had patient informed consent and were histologically confirmed. The protocol used to obtain the tissues was approved by the Sant'Andrea Hospital Bioethical Committee. Each tumor was paired with a non-tumor gastric mucosal control from the same patient.

[0192] Microarray:

[0193] Microarray analysis was performed as described (Liu et al., 2004). Briefly, 5ug of total RNA was used for hybridization on a generation 2 miRNA microarray chip (V2). These chips contain gene-specific 40-mer oligonucleotide probes for 250 human miRNAs spotted by contact technology and covalently attached to a polymer matrix. Hybridize the microarray in 6X SSPE (0.9M NaCl_60mM NaH2PO4_H2O_8mM EDTA, pH 7.4), 30% formamide for 18 hours at 25°C and wash in 0.75X TNT (Tris / HCl / NaCl / Tween 20) at 37°C 40 min and t...

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Abstract

Methods and compositions for the diagnosis, prognosis and / or treatment of gastric cancer associated diseases are disclosed.

Description

[0001] Inventors: Carlo M. Croce, Fabio Petrocca, Andrea Vecchione [0002] Cross Reference Related Applications [0003] This application claims the benefit of US Provisional Application 61 / 067,445, filed February 28, 2008, the entire disclosure of which is expressly incorporated herein by reference. [0004] Statement Regarding Federally Sponsored Research [0005] This invention was made with government support under NCI grants CA76259 and CA8134. The Government has certain rights in this invention. [0006] Technical Field and Industrial Applicability of the Invention [0007] The present invention relates generally to the field of molecular biology. Certain aspects of the invention include use in the diagnosis, treatment and prognosis of gastric cancer related conditions. Background of the invention [0008] There is no admission that the background art disclosed in this section legally constitutes prior art. [0009] Although the incidence of gastric cancer decline...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/136C12Q2600/106C12Q2600/112C12Q2600/178C12Q1/6886A61P1/04A61P35/00C12N15/113C12N2310/141C12Q2525/205
Inventor C·M·克罗斯F·派特罗卡A·维克驰欧尼
Owner THE OHIO STATE UNIV RES FOUND
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