Method for extracting refined tea saponin from camellia oleifera seed cake
A technology of tea saponin and camellia oleifera seed cake, which is applied in chemical instruments and methods, preparation of sugar derivatives, steroids, etc., can solve defects in process, purity, yield or cost, restrict the application of tea saponin, and cause eye damage Large and other problems, to achieve the effect of low cost, low production cost, good physical and chemical stability
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Embodiment 1
[0036] Weigh 190 g of pulverized camellia seed cake, extract with 950 mL of 85% ethanol under reflux, and extract continuously for 2 times, each time for 3 hours. The extracts were combined, concentrated under reduced pressure, ethanol was recovered, and the concentrated solution was diluted with water to a ratio of solid to liquid of 1:5. In a water bath at 50°C, add 55 mL of chitosan solution, stir thoroughly, and after standing for 3 hours, filter the solution with suction to remove the precipitate to obtain a transparent and clear liquid. Weigh 213g of pretreated AB~8 macroporous adsorption resin and put it into a glass column. The transparent and clear liquid is first fully absorbed by AB~8 macroporous adsorption resin, and then the impurities are eluted with 0.05% sodium hydroxide solution and used to remove Wash with ion water until the effluent is neutral. Then elute with 70% ethanol until no tea saponin is detected in the effluent, and this part of the eluate is coll...
Embodiment 2
[0038] Weigh 200 g of pulverized Camellia oleifera seed cake, extract with 1200 mL of 85% ethanol under reflux, and extract continuously for 2 times, each time for 2.5 hours. The extracts were combined, concentrated under reduced pressure, ethanol was recovered, and the concentrated solution was diluted with water to a ratio of solid to liquid of 1:6. In a water bath at 60°C, add 55mL of chitosan solution, stir well, let stand for 2 hours, filter the solution with suction, remove the precipitate, and obtain a transparent and clear liquid. Weigh 214g of pretreated AB~8 macroporous adsorption resin and put it into a glass column. The transparent and clear liquid is first fully absorbed by AB~8 macroporous adsorption resin, and then the impurities are eluted with 0.2% sodium hydroxide solution. Wash with ion water until the effluent is neutral. Then elute with 70% ethanol until no tea saponin is detected in the effluent, and this part of the eluate is collected. Add hydrogen pe...
Embodiment 3
[0040] Weigh 200 g of pulverized Camellia oleifera seed cake, extract with 800 mL of 85% ethanol under reflux, and extract continuously for 3 times, each time for 2 hours. The extracts were combined, concentrated under reduced pressure, ethanol was recovered, and the concentrated solution was diluted with water to a ratio of solid to liquid of 1:5. In a water bath at 50°C, 60 mL of chitosan solution was added, fully stirred, and allowed to stand for 2.5 hours, and the solution was suction-filtered to remove precipitates to obtain a transparent and clear liquid. Weigh 213g of pretreated AB~8 macroporous adsorption resin and put it into a glass column. The transparent and clear liquid is first fully absorbed by AB~8 macroporous adsorption resin, and then the impurities are eluted with 0.05% sodium hydroxide solution and used to remove Wash with ion water until the effluent is neutral. Then elute with 80% ethanol until no tea saponin is detected in the effluent, and this part of...
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