Hypsizigus marmoreus mon-mon cross-breeding method

A technology of hybrid breeding and shiji mushroom, which is applied in the field of high-efficiency jiji mushroom single-cross breeding, can solve the problem of low efficiency of shiji mushroom strain selection and achieve the effect of improving breeding efficiency

Inactive Publication Date: 2011-05-11
SHANGHAI FINC BIO TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0013] Because the mushroom has a good market, some companies in China, Japan and South Korea have begun to carry out factory cultivation in an ar

Method used

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  • Hypsizigus marmoreus mon-mon cross-breeding method

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Embodiment Construction

[0056] In order to make the technical means, creative features, goals and effects of the present invention easy to understand, the present invention will be further described below in conjunction with specific illustrations.

[0057] A kind of true jitake mushroom single hybridization breeding method, such as figure 1 As shown, including:

[0058] (1) Selection of parents: Select the appropriate Hypsizygus marmoreus parent F-W (white) and parent F-4 (brown) according to the breeding objectives, familiarize yourself with the characteristics of each parent, and be familiar with its advantages and disadvantages. kinship, have good synergy;

[0059] (2) Spore collection, spore dilution coating, single spore picking, microscopic examination of lock joints to obtain monokaryotic hyphae; no lock joints are monokaryotic mycelia;

[0060] Test tube, plate medium, Erlenmeyer flask medium: use PDA medium. The spore collection mainly adopts the hook suspension method to collect spores;...

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Abstract

The invention relates to a hypsizigus marmoreus mon-mon cross-breeding method which comprises the steps of: selecting a parent; collecting spores, diluting and coating the spores, selecting single spores, and microscopically examining clamp connection free spores as monocaryon myceliums; diluting and coating the spores in a sterile operation manner, coating by using the concentration that 2-3 spores are in 40 times micro view, and culturing for 8-10 days at a temperature of 16-21 DEG C; selecting single spores in sparse parts; combining and hybridizing monocaryon myceliums: carrying out pairing hybridization on the parent of the monocaryon myceliums, and carrying out pairing hybridization by selecting pure white monocaryon myceliums with high spawn running speed and dense myceliums; microscopically examining monocaryon myceliums with clamp connection, and then selecting heterozygotes; microscopically examining the heterozygotes in a 63 times view, searching 3-5 views in different regions, and if the clamp connection exists, enabling the monocaryon myceliums to be heterozygotes; cultivating and fruiting; and carrying out pilot scale test and large scale test, and popularizing. In the method, the breeding time is reduced to 18 months from several years, and a plurality of bacterial strains with high yield, excellent quality and strong stress tolerance are obtained.

Description

technical field [0001] The invention relates to a method for hybrid breeding of fungi, in particular to a method for high-efficiency single hybrid breeding of Hypsizygus marmoreus. The whole process and operation requirements of the single hybrid breeding method of jijiji mushroom are disclosed, including the required culture medium, selection of parents, hybridization process and technology, cultivation and fruiting. Background technique [0002] Hypsizigus marmoreus is also known as Yuqin, Banyuqin, Seafood Mushroom, Jiaoyu Mushroom, Hongxi Mushroom, Crab-flavored Mushroom, False Matsutake and so on. Belonging to Basidiomycotina, Phyllomycetes, Agaricaceae, Agaricaceae, Liphyllum family, and Amygium genus, it is an excellent edible fungus in the northern temperate zone. The taste of shimeji mushroom is fresher than that of oyster mushroom, the meat is thicker than that of shiitake mushroom, and the quality is tougher than that of shiitake mushroom. [0003] Shiji mushroo...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H15/00
Inventor 程继红刘玉陈传喜
Owner SHANGHAI FINC BIO TECH INC
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