Human sperm DNA fragment detection method and detection kit

Abstract

The invention provides a human sperm DNA fragment detection method comprising the following steps: (1) diluting liquefied sperm with a buffer solution of 4 DEG C; (2) adding the diluted sperm into a sample tube of a flow cytometry; (3) adding an acid treatment buffer solution; (4) after 30 seconds, adding an AO staining solution; (5) setting a calibration and flow chamber of the flow cytometry, equilibrating a sample line by an AO equilibration buffer solution; (6) continuously determining each sample at least twice, recording at least 5000 cells and carrying out statistical analysis; and (7) after determining, respectively thoroughly cleaning the cell fragments and fluorescent dye remaining in the sample line of the flow cytometry by bleacher, sample tube detergent and degerming double distilled water. A human sperm DNA fragment detection kit comprises 50mL of staining buffer solution, 300mu L of AO stock solution, 20mL of acid treatment solution, 100mL of buffer solution, 100mL of sheath fluid, 100mL of AO remover, 100mL of sample tube detergent and 100mL of double distilled water (ddH2O). The invention realizes the purpose of clinically detecting the sperm DNA fragments simply, conveniently, quickly and accurately.

Description

technical field [0001] The invention relates to reagents used in clinical medicine, in particular to a detection method and a detection kit of human sperm DNA fragments. Background technique [0002] With the development of the economy and the improvement of the medical level, the incidence of infertility is increasing, and has become a major disease that affects human life and health today. Among them, in my country, the incidence of infertility is in the More than 10%, male factors and female factors account for 30% each, and the common factors of both parties account for 40%. Among the male factors, the cause of sperm ranks first. The integrity of DNA, the carrier of sperm genetic material, can affect the fertilization ability of sperm, the division of fertilized eggs and the normal development of embryos. Fragmentation of sperm DNA can lead to infertility, repeated miscarriages and assisted pregnancy Treatment success rate is low. Therefore, the structural analysis of s...

AI Technical Summary

Problems solved by technology

Among the male factors, the cause of sperm ranks first. The integrity of DNA, the genetic material carrier of sperm, can affect the fertilization ability of sperm, the division of fertilized eggs and the normal development of embryos. Fragmentation of sperm DNA can lead to infertility, repeated miscarriage and assisted pregnancy low success rate of treatment