Clathrate compound formed by Salvia miltiorrhiza Bunge and cyclodextrin and application thereof
A cyclodextrin and clathrate technology, which is applied in the field of inclusion complexes formed by Dan participating in cyclodextrin derivatives and their preparation
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Embodiment 1
[0023] At room temperature, treat 200 mg of Salvia miltiorrhiza in 20 ml of 40% hydroxypropyl-β-cyclodextrin for about 4 hours to obtain a slightly cloudy solution, which is filtered with a 0.2 μ microporous membrane until it becomes clear. The concentration of Salvia miltiorrhiza in the solution can be Up to 8-10mg / ml (the detection method is HPLC, and the detector is an evaporative light scattering detector). The solution was filled in ampoules, and the quality remained unchanged for 18 months after sterilization.
Embodiment 2
[0025] At room temperature, dissolve 30 g of hydroxypropyl-β-cyclodextrin in 80 ml of water, and treat 300 mg of Salvia miltiorrhiza for about 3 hours to obtain a slightly cloudy solution. Filter it with a 0.2 μ microporous membrane until it becomes clear, and take the clear solution Freeze-dry to obtain a white powder, which can be redissolved in water. It can be proved by differential scanning calorimetry that the white powder is in the form of clathrate, and the clathrate rate is 90%.
Embodiment 3
[0027] 8 mg of Salvia miltiorrhiza and 76 mg of β-cyclodextrin were fully ground in 50% ethanol 5 until a uniform paste was formed, and a white powder was obtained after vacuum drying.
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