Unlock instant, AI-driven research and patent intelligence for your innovation.

Pharmaceutical composition for osteoarthritis molding and preparation method thereof as well as molding method

A technology for osteoarthritis and composition, which is applied to the field of pharmaceutical composition for osteoarthritis modeling and its preparation, can solve the problems of easy diffusion, long molding time, complicated operation, etc., and achieves fast and successful molding High rate and simple method

Inactive Publication Date: 2012-08-22
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the commonly used modeling methods for osteoarthritis are not ideal, and most of them are surgical modeling. The main disadvantages of this method are that the operation is complicated and the trauma is large. The curative effect of the drug; the main disadvantage of the method of intra-articular injection of the compound, one is that it needs to be injected three times repeatedly to form a mold, which takes a long time and is uncertain
The second is that after being prepared with normal saline, the liquid medicine has fluidity, and after entering the joint, it cannot adhere to the articular surface and only destroys the cartilage tissue, but is easy to spread to the joint capsule wall, causing serious damage to the soft tissue around the joint, causing acute inflammation and obvious inflammation. Swelling makes it difficult to find a position for re-injection, affects the accuracy of dosage and the consistency of pathological damage, and has a high mortality rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pharmaceutical composition for osteoarthritis molding and preparation method thereof as well as molding method
  • Pharmaceutical composition for osteoarthritis molding and preparation method thereof as well as molding method
  • Pharmaceutical composition for osteoarthritis molding and preparation method thereof as well as molding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A pharmaceutical composition for osteoarthritis modeling, consisting of papain, poloxamer and PH 5.7 phosphate buffer, the concentration (g / 100ml) of papain 4% and poloxamer according to its weight to volume ratio Sham 35%, the rest is phosphate buffer.

[0033] The preparation method of the above-mentioned pharmaceutical composition for osteoarthritis modeling, the specific steps are:

[0034] (1) Add 35g of poloxamer to every 100ml of pH5.7 phosphate buffer and mix well;

[0035] (2) Continue to add 4g papain poloxamer and stir evenly.

[0036] The above-mentioned modeling method of the pharmaceutical composition used for osteoarthritis modeling, the specific steps are:

[0037] (1) Take the above pharmaceutical composition and pre-cool it in a refrigerator at 4°C for 30 minutes, then take it out;

[0038] (2) The syringe draws 0.1 ml of the pharmaceutical composition in step (1), and injects it into the knee joint cavity of the modeled rat;

[0039] (3) The injec...

Embodiment 2

[0042] A pharmaceutical composition for osteoarthritis modeling, consisting of papain, poloxamer and PH 5.7 phosphate buffer, the concentration (g / 100ml) of papain 3.5% and poloxamer according to its weight to volume ratio Sham 35%, the rest is phosphate buffer.

[0043] The preparation method of the above-mentioned pharmaceutical composition for osteoarthritis modeling, the specific steps are:

[0044] (1) Add 35g of poloxamer to every 100ml of pH5.7 phosphate buffer and mix well;

[0045] (2) Continue to add 3.5g of papain and stir evenly.

[0046] The above-mentioned modeling method of the pharmaceutical composition used for osteoarthritis modeling, the specific steps are:

[0047] (1) Take the above-mentioned pharmaceutical composition and put it in a refrigerator at 5°C for 20 minutes, then take it out;

[0048] (2) The syringe draws 0.05ml of the pharmaceutical composition in step (1), and injects it into the rabbit knee joint cavity;

[0049] (3) Place the injected ...

Embodiment 3

[0052] A pharmaceutical composition for osteoarthritis modeling, consisting of papain, poloxamer and PH 5.7 phosphate buffer, the concentration (g / 100ml) of papain 3%, poloxamer according to its weight to volume ratio Sham 30%, the rest is phosphate buffer.

[0053]The preparation method of the above-mentioned pharmaceutical composition for osteoarthritis modeling, the specific steps are:

[0054] (1) According to every 100ml of pH5.7 phosphate buffer, add 3g of papain and mix well;

[0055] (2) Continue to add 30g of poloxamer and stir evenly.

[0056] The above-mentioned modeling method of the pharmaceutical composition used for osteoarthritis modeling, the specific steps are:

[0057] (1) Take the above pharmaceutical composition and pre-cool it in a refrigerator at 3°C ​​for 40 minutes, then take it out;

[0058] (2) The syringe draws 0.07ml of the pharmaceutical composition in step (1), and injects it into the knee joint cavity of the rat

[0059] (3) The injected rat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a pharmaceutical composition for osteoarthritis molding and a preparation method thereof as well as a molding method. The pharmaceutical composition comprises the following components by weight-to-volume ratio concentration (g / 100 mL): 3-5% of papain, 30-35% of poloxamer, and the balance of a phosphate buffer solution with the PH of 5.7. The pharmaceutical composition for osteoarthritis molding utilizes the dual properties of controlled release and gelatinization of poloxamer, so that papain injected into joints does not have fluidity, is attached on the surface of cartilage with the gelatinous poloxamer, and releases to cartilage to directly cause cartilage damage to obtain an osteoarthritis model. The method is simple to operate, has high molding speed and high success ratio, and is suitable for the pharmacodynamic study of medicines for treating osteoarthritis.

Description

technical field [0001] The invention relates to a modeling pharmaceutical composition, a preparation method and a modeling method thereof, in particular to a pharmaceutical composition for osteoarthritis modeling, a preparation method and a modeling method. Background technique [0002] At present, the commonly used modeling methods for osteoarthritis are not ideal, and most of them are surgical modeling. The main disadvantages of this method are that the operation is complicated and the trauma is large. The curative effect of medicine; The main disadvantage of the method of intra-articular injection of compound, one is that it needs repeated 3 injections to form a mold, which takes a long time and is uncertain. The second is that after being prepared with normal saline, the liquid medicine has fluidity, and after entering the joint, it cannot adhere to the articular surface and only destroys the cartilage tissue, but it is easy to spread to the joint capsule wall, causing s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/48A61K47/34A61K9/06A61K47/10
Inventor 柳占彪崔元璐胡利民高秀梅师咏梅
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com