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Low temperature resistant methane bacterium and preparation method thereof

A low-temperature, biogas-resistant technology, applied in bacteria, fungi, waste fuels, etc., can solve the problems of low gas production in biogas digesters, increased investment in user infrastructure, and non-functioning biogas digesters.

Inactive Publication Date: 2011-05-25
段开红 +2
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is greatly affected by the ambient temperature, especially in winter in the northern region, the gas production of the biogas digester is small, or there is no gas production, which makes the biogas digester not work normally.
Most of the measures taken are to thicken and insulate the biogas digesters, or adopt measures such as solar radiation heating and heat preservation rings, etc. The disadvantages are that it increases the user's infrastructure investment, and the transformation of old biogas water also presents infrastructure difficulties and access Factors such as inconvenient materials

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0016] Select 10 samples from Inner Mongolia, Hebei, Shanxi, Yinchuan and other areas in winter, and culture them in the medium of acetic acid bacteria, butyric acid bacteria, lactic acid bacteria, yeast, and methane bacteria, respectively, and select the respective strains by microscopic examination and observation in the medium. Inject the respective strains with 1.5% sucrose, 6% bean cake powder, 3.2% peptone, 0.8% beef extract, 0.5% mgSO 4 ·7H 2 O, 0.04% (NH 4 ) 2 SO 4 、0.02KH 2 PO 4 and 0.02% Na 2 HPO 4 medium. The inoculum size is 5% pH6.5-7.0, and the culture temperature is 15°C, 13°C, 11°C, 9°C, 8°C, 7°C, 6°C, 5°C, and 4°C.

[0017] Stop the inoculation culture when the proliferation is less than 30%, and reach the minimum temperature of yeast at 5°C, the minimum temperature of lactic acid bacteria at 3°C, the temperature of acetic acid bacteria at 4°C, the temperature of butyric acid bacteria at 8°C, and the temperature of methane bacteria at 7°C.

[0018] Pu...

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PUM

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Abstract

The invention discloses a low temperature resistant methane bacterium and a preparation method thereof and the methane bacterium effectively solves the defect that the gas output of the methane tank is low in winter. The preparation method of the low temperature resistant methane bacterium comprises the following steps: sampling and breeding five strains, namely acetic acid bacteria, lactic acid bacteria, butylic acid bacteria, saccharomycetes and methane bacteria from a northern methane tank in winter, performing low temperature breeding, performing pure solid culture and mixing in a ratio according to the number of microbial floras, which is suitable for methane production. The bacterium is bred and the preparation method is simple and cheap; by using the bacterium, the winter utilization rate of the northern methane tank can be effectively increased, the popularity of methane in rural areas can be extremely increased, the quality of life of rural population can be improved and the rural environment can be improved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, in particular to a preparation method of a composite microbial fermentation agent. Background technique [0002] At present, most of the microorganisms used in biogas production adopt methods such as natural fermentation or inoculation of biogas slurry and biogas residue. This method is greatly affected by the ambient temperature, especially in winter in the northern region, the gas production of the biogas digester is small, or there is no gas production, which makes the biogas digester not work normally. Most of the measures taken are to thicken and insulate the biogas digesters, or adopt measures such as solar radiation heating and heat preservation rings, etc. The disadvantages are that it increases the user's infrastructure investment, and the transformation of old biogas water also presents infrastructure difficulties and access Inconvenient materials and other factors. Co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12P5/02
CPCY02E50/343Y02E50/30
Inventor 段开红刘和仁王君喜
Owner 段开红
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