Low temperature resistant methane bacterium and preparation method thereof
A low-temperature, biogas-resistant technology, applied in bacteria, fungi, waste fuels, etc., can solve the problems of low gas production in biogas digesters, increased investment in user infrastructure, and non-functioning biogas digesters.
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[0016] Select 10 samples from Inner Mongolia, Hebei, Shanxi, Yinchuan and other areas in winter, and culture them in the medium of acetic acid bacteria, butyric acid bacteria, lactic acid bacteria, yeast, and methane bacteria, respectively, and select the respective strains by microscopic examination and observation in the medium. Inject the respective strains with 1.5% sucrose, 6% bean cake powder, 3.2% peptone, 0.8% beef extract, 0.5% mgSO 4 ·7H 2 O, 0.04% (NH 4 ) 2 SO 4 、0.02KH 2 PO 4 and 0.02% Na 2 HPO 4 medium. The inoculum size is 5% pH6.5-7.0, and the culture temperature is 15°C, 13°C, 11°C, 9°C, 8°C, 7°C, 6°C, 5°C, and 4°C.
[0017] Stop the inoculation culture when the proliferation is less than 30%, and reach the minimum temperature of yeast at 5°C, the minimum temperature of lactic acid bacteria at 3°C, the temperature of acetic acid bacteria at 4°C, the temperature of butyric acid bacteria at 8°C, and the temperature of methane bacteria at 7°C.
[0018] Pu...
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