Method for preparing microporous membrane for transmittance analysis

A microporous film, Fanghua technology, applied in semi-permeable membrane separation, chemical instruments and methods, membrane technology, etc., can solve the problems of low sample success rate and harsh requirements, and achieve high sample success rate and simple preparation process. Effect

Inactive Publication Date: 2011-05-25
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few literatures on the preparation of microporous films, and the preparation environmental factors (such as laboratory temperature, humidity, etc.) are demanding, and the success rate of samples is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Dissolve Fanghua powder (polyvinyl formal) in chloroform solution (stand still for 3-5 days) to prepare Fanghua solution with a mass percentage concentration of 0.1-0.3%, add the above-mentioned Fanghua solution with a volume of 1-3% Surfactant (non-ionic surfactant, HLB value 1.5 ~ 1.8, such as Span 85), add glycerin under strong stirring, after the glycerin is dispersed, it will be distributed on the surface of Fanghua film in the form of star droplets to form various For a pore-like structure of one scale, the amount of glycerin added is 3-5% of the volume of the Fanghua solution, stirred for 30 minutes, and left to stand for use.

[0015] Immerse the glass slides or long glass strips in concentrated sulfuric acid, deionized water and absolute ethanol in order to clean them, and immerse the surface-cleaned glass slides or long glass strips in the prepared Fanghua solution, vertically or with Lift up at a small angle in the vertical direction (note: the angle and spee...

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Abstract

The invention provides a method for preparing a microporous membrane for transmittance analysis, comprising the following steps: (1) dissolving polyvinyl formal in a chloroformic solution to obtain a polyvinyl formal solution with the concentration of 0.1-0.3wt%; (2) adding a surface active agent which accounts for 1-3% of the total solution volume, and adding a pore forming agent under stirring; (3) soaking a glass slide into the polyvinyl formal solution prepared in the step 2, taking out the soaked glass slide, soaking the glass slide in an organic solvent again after the surface of the glass slide is dried, and then forming controllable pores with even size and distribution on the surface of a polyvinyl formal membrane; (4) stretching the polyvinyl formal membrane on a deionized water surface, laying a metal grid on the polyvinyl formal membrane, laying filter paper on the metal grid, and lifting the water-containing surface of the filter paper and drying after the filter paper is soaked completely to obtain a grid, wherein a layer of porous polyvinyl formal membrane is adhered to the surface of the grid; and (5) depositing a layer of carbon on the surface of the polyvinyl formal membrane.

Description

technical field [0001] The invention relates to a preparation method of a microporous film, in particular to a preparation method of a microporous film for transmission analysis. Background technique [0002] Transmission electron microscopy has become an indispensable research tool in the field of material science and life science research, and its sample support tool - micro-grid film (support film) has become one of the key factors affecting the success of sample observation. Micro-grid film is deliberately made micro-holes on micro-porous organic film (commonly known as Fang Hua film). Micropores have different shapes and sizes ranging from 0.25 μm to 5 μm. After carbon spraying, there is no residual film layer on the micropores, which are through holes. The microgrid membrane is very strong and can support a variety of samples. Microgate membranes can be used from large crystalline samples to small virus particles. These samples are carried on the micro-grid membrane...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01J37/20B01D67/00B01D69/06
Inventor 吕振刚李飞燕小飞杨春鹏李茹包艳
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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