Method for transplanting Damascus rose tissue culture seedling
A technology for tissue culture seedlings and roses, which is applied in the field of plant tissue cultivation, can solve the problems of long rooting time, low survival rate, and decline in the quality of offspring, and achieve the effect of good growth and improved survival rate
Active Publication Date: 2013-02-20
DASHUN INT FLOWER
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Problems solved by technology
Its propagation method is mainly cutting propagation, the survival rate is low, the rooting time is long, and the quality of offspring is reduced
At present, plant tissue culture methods are also used for propagation, but the survival rate of tissue culture seedlings is low, which greatly limits the factory production of Damask roses
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[0007] This experiment is divided into test group and control group. 500 tissue-cultured seedlings were selected as experimental materials for each treatment of the test group and the control group.
[0008] Experimental method of the experimental group: After the Damascus rose tissue culture seedlings were hardened in the greenhouse for one week, the tissue culture seedlings were taken out from the culture bottle, the root medium was washed with clean water, and directly transplanted on the plug tray equipped with the transplanting medium. The planting medium is a mixed matrix of peat, river sand, and perlite, and its volume ratio is shown in Table 1. After the tissue culture seedlings are planted, 0.1% (volume to volume ratio) carbendazim aqueous solution is poured once, and then poured once every 5 days, continuously poured 3 times, and the seedbed is covered with a film. The cultivation temperature is 20-23°C, the humidity is 90%-95%, the shading is 60-70%, the light is 6...
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The invention relates to a method for transplanting a Damascus rose tissue culture seedling, comprising the following steps of: hardening off the Damascus rose tissue culture seedling for a week; then taking out the tissue culture seedling from a culture bottle; cleaning a root culture medium with clear water; directly transplanting the root culture medium into a hole disc filled with a transplanting substrate; setting and then irrigating a 0.1% carbendazol water solution once; irrigating again every other 5 days; continuously irrigating three times; covering a film on a seed bed, and uncovering the film every day for ventilating, wherein the culture temperature is 20-23 DEG C, the humidity is 90-95%, the shading light rate is 60-70%, and the illumination is 6,000-8,000Lx; irrigating an MS (Murashige and Skoog) culture medium which is diluted ten times every other three days once to promote the root system growth and the leaf germination; and after new leaves are grown, reducing the humidity to 70-80%, enhancing the illumination to 8,000-12,000 Lx and controlling the culture temperature to be 23-28 DEG C.
Description
technical field [0001] The invention relates to the field of plant tissue cultivation methods; in particular to the field of Damask rose cultivation methods. Background technique [0002] Rosa damascena is a shrub. It is native to Asia Minor and has been cultivated in southern Europe for a long time. It is used as raw material for incense making and has high economic value. It has been introduced and cultivated in various parts of our country in recent years for viewing and extracting essence. Its propagation method is mainly cutting propagation, the survival rate is low, the rooting time is long, and the quality of offspring declines. At present, the plant tissue culture method is also used for propagation, but the transplanting survival rate of tissue culture seedlings is low, which greatly limits the factory production of Damask rose. Contents of the invention [0003] In order to solve the technical problems of long propagation period and low survival rate, the pres...
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IPC IPC(8): A01H4/00A01G31/00
Inventor 杨铁顺
Owner DASHUN INT FLOWER