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Chemiluminescence quantitative detection kit for free triiodothyronine

A technology of triiodothyronine chemistry, which is applied in the field of free triiodothyronine chemiluminescence quantitative detection kits, can solve the problems of complicated operation, high price, and difficulty in popularization.

Inactive Publication Date: 2011-06-15
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign automatic chemiluminescent enzyme immunoassay analyzers and matching kits are mostly used in closed systems, which are expensive and complicated to operate, and are not easy to promote in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Preparation of FT3 quantitative detection kit

[0018] (1) Anti-FT3 antibody-coated plate preparation

[0019] a. Coating: Take 1mol / L Na 2 HPO 4 77.4ml and 1mol / L NaH 2 PO 4 22.6ml and mix well, add deionized water to 1000ml to form a 10-fold coating solution, dilute ten times before use, add an appropriate amount of anti-FT3 monoclonal antibody (purchased from fitzgerald) and mix well, then add to the wells of the microplate medium, 100μl / well, 4°C for 16 hours;

[0020] b. Sealing: Discard the coating solution, pat dry on absorbent paper, add 3% BSA and 0.05% preservative (Proclin TM 300) of phosphate buffer (pH 7.4), 200 μl / well, 37 ° C for 2 hours;

[0021] c. Seal the bag: Discard the sealing solution, pat it dry on absorbent paper, pump it in a vacuum oven for 5 hours at room temperature, immediately vacuum seal the bag, check for air leakage, and re-seal the bag if there is a label. Store at 2-8°C.

[0022] (2) Preparation of enzyme marke...

Embodiment 2

[0037] Example 2: The method of using the FT3 chemiluminescence quantitative detection kit

[0038] 1. Reagent and Sample Preparation

[0039] (1) Reagent preparation

[0040] a. Equilibrate the kit at room temperature (18-26°C) for 20 minutes.

[0041] b. Take out the concentrated washing solution from the kit, dilute it 1:20 with fresh purified water and add it to the washing solution bottle of the plate washer.

[0042] (2) Sample preparation

[0043] The qualified serum or plasma to be tested should be equilibrated at room temperature (18-26° C.) for 20 minutes before use.

[0044] 2. Operation steps

[0045] a. Take out the required FT3 antibody-coated microwell plate and place it on the microwell rack

[0046] b. Add calibrators 1-6, quality control substances 1-2 and samples to be tested, 50 μl per well.

[0047] c. Add enzyme marker 100 μl / well.

[0048] d. Gently oscillate to mix.

[0049] e. Incubate at room temperature for 1 hour.

[0050] f. Discard the wast...

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PUM

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Abstract

The invention discloses a chemiluminescence quantitative detection kit for free triiodothyronine. The kit comprises a free triiodothyronine detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with a free triiodothyronine antibody. The kit can specifically and quantitatively detect the content of the free triiodothyronine in patient serum, and is used for the auxiliary diagnosis of thyroid dysfunction diseases. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical testing and chemiluminescence immunoassay, and specifically relates to a chemiluminescent quantitative detection kit for free triiodothyronine (FT3) and related preparation methods and usage methods. Background technique [0002] Thyroid dysfunction is the most common endocrine disease, and it is still easily underdiagnosed. According to the census of 46,000 people in 11 cities in the United States, it is found that about 11% of the people have various thyroid dysfunctions that have not been diagnosed. Thyroid dysfunction can be divided into hyperthyroidism (referred to as hyperthyroidism) and hypothyroidism (hypothyroidism, referred to as hypothyroidism). [0003] Thyroid hormone is the collective name for thyroxine (T4) and triiodothyronine (3,5,3'-triiodothyronine, T3), both of which are iodine-containing derivatives of tyrosine. More than 99% of T3 and T4 in plasma are reversibly b...

Claims

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Application Information

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IPC IPC(8): G01N33/78G01N33/543G01N33/545G01N33/535G01N21/76
Inventor 穆海东汪宁梅穆宇豪王通
Owner 上海裕隆生物科技有限公司
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