Aflatoxin M1 immunochromatographic test strip and preparation method thereof
An immunochromatographic test strip and aflatoxin technology, which is applied in analytical materials, measuring devices, instruments, etc., can solve problems such as the advent of aflatoxin M1 immunochromatographic test strips, and achieve the effect of simple operation and high detection sensitivity.
Inactive Publication Date: 2011-06-29
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology
However, there is no immunochromatographic test strip for aflatoxin M1 in the world at present.
Method used
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Embodiment 2
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Abstract
The invention belongs to the field of biological detection. An aflatoxin M1 immunochromatographic test strip is characterized by comprising a paper board, wherein a water absorption pad, a detection pad, a gold-labeled pad and a sample pad are attached to one side of the paper board in turn from top to bottom, wherein the adjacent pads are overlapped at joints; the detection pad takes a nitrocellulose membrane as a base pad; the nitrocellulose membrane is provided with a transverse quality control line and a transverse detection line from top to bottom; the detection line is coated with an aflatoxin M1-bovine serum albumin (AFM1-BSA) conjugate; the quality control line is coated with a rabbit anti-mouse polyclonal antibody; a nanogold-labeled aflatoxin M1-resistant monoclonal antibody is transversely sprayed on the gold-labeled pad; and the aflatoxin M1-resistant monoclonal antibody is produced by a hybridoma cell strain 2C9 with the collection number of CCTCCNO.C201018. The test strip is used for detecting aflatoxin M1 and has the characteristics that: the test strip quickly detects the aflatoxin M1, is easy to operate and has high sensitivity.
Description
Aflatoxin M1 immunochromatographic test strip and preparation method thereof technical field The invention belongs to the field of biological detection, and in particular relates to an aflatoxin M1 immunochromatographic test strip and a preparation method thereof. Background technique Aflatoxin is mainly a secondary metabolite secreted by Aspergillus flavus and Aspergillus parasiticus, and is a natural toxic compound that can cause various damages to humans and animals. More than 20 types of aflatoxin (AFT) have been discovered, among which aflatoxin B1 is the most toxic and carcinogenic substance. Aflatoxin M1 is a hydroxylated metabolite of aflatoxin B1 and is also a strong carcinogen. When mammals ingest AFB1-contaminated feed, AFM1 is secreted into milk through hydroxylation in vivo. Aflatoxin M1 is present in milk produced by dairy cows fed feed contaminated with aflatoxin B1. Since aflatoxin M1 is quite stable, it cannot be killed by pasteurization, so the detecti...
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Login to View More IPC IPC(8): G01N33/558G01N33/532
Inventor 李培武张道宏张奇张文管笛丁小霞姜俊
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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