Methods for separating leucine and arginine by selective precipitation

Abstract

The invention discloses a method for separating leucine by selective precipitation first, which comprises the following steps: adding dichlorobenzenesulfonic acid into hair acid hydrolysis solution, wherein the weight / volume ratio of the dichlorobenzenesulfonic acid to the hydrolysis solution is 1:(5-20); stirring the hair acid hydrolysis solution; and separating a generated leucine precipitate to obtain leucine. The invention also discloses a method for separating arginine by selective precipitation, which comprises: supplying dichlorobenzenesulfonic acid to the hydrolysis solution from which the leucine precipitate is separated according to a certain ratio; continuously stirring for 12 to 36 hours; and separating a generated arginine precipitate to obtain the arginine. The invention also discloses the preparation method of the dichlorobenzenesulfonic acid used in the methods for separating leucine and arginine by selective precipitation. In the invention, the methods are simple, the operation is convenient, the precipitation rate is high, and the leucine and arginine yields (relative to weight of hair) are high.

Description

technical field [0001] The invention relates to the field of amino acid production, in particular to a method for selective precipitation and separation of leucine and arginine. Background technique [0002] The production of amino acids by extraction is the earliest amino acid production method, and it is also a very important amino acid production method. The basic procedures of this method are the same. First, it is necessary to select a suitable protein as a raw material, and then use an appropriate method to hydrolyze the protein into a mixed amino acid, and then use a suitable separation method to separate and purify the mixed amino acid to extract the target protein. amino acid. [0003] The technical key and technical difficulty of the extraction method mainly lie in how to selectively separate the target amino acid from the mixed amino acid solution containing more than a dozen amino acids. The current method for extracting amino acids from hair can be extracted f...

AI Technical Summary

Problems solved by technology

[0005] The main shortcoming of above-mentioned technology is: (1) technological process is long, and technology is more complicated; (2) the yield of leucine, arginine is on the low side, generally only has 1~3%; (3) primary mother liquor and secondary mother liquor After extracting arginine and leucine respectively, they cannot be well utilized, resulting in waste of amino acid resources and serious environmental pollution.