Human miR-185* antisense nucleic acid and application thereof

An antisense and nucleotide technology, applied in the field of biomedical materials, can solve the problems of poor curative effect of patients with distant metastasis, low survival rate of middle and advanced patients, and low survival rate, and achieve the ability of inhibiting growth and malignant proliferation Effect

Inactive Publication Date: 2011-08-03
SUZHOU GENEPHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Human miR-185* antisense nucleic acid and application thereof
  • Human miR-185* antisense nucleic acid and application thereof
  • Human miR-185* antisense nucleic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1, miR-185 * Detection of antisense oligonucleotides' inhibitory activity on human glioma cell line U87 / MG

[0033] First, miR-185 was synthesized by Shanghai Jima Pharmaceutical Technology Co., Ltd. * Antisense oligonucleotide, the sequence is: 5'-GACCAGAGGAAAGCCAGCCCCU-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0034] Cell culture:

[0035] U87 / MG cells (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences), cultured in 10% FBS-DMEM medium (FBS was purchased from Gibco, DMEM was purchased from Hyclone), 37 ° C, 5% CO 2 to cultivate. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0036] Transfection:

[0037] 1) One day before transfection, inoculate cultured cells in a 96-well plate with an appropriate amount of culture m...

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Abstract

The invention discloses antisense oligonucleotide inhibiting human microRNA-185* from expressing and application thereof. The antisense oligonucleotide is specifically combined with human miR-185*, contains a sequence complementary with at least 13 continuous nucleotides in a 5'-AGGGGCUGGCUUUCCUCUGGUC-3' nucleotide sequence, particularly a sequence of 5'-GACCAGAGGAAAGCCAGCCCCU-3'. The antisense oligonucleotide can be ribonucleotide, deoxyribonucleotide or chimera of both, and can modify any nucleotide in a chain. The miR-185* antisense oligonucleotide effectively inhibits miR-185* in a human glioma cell from expressing, and inhibits the cell from growing and propagating so as to effectively treat human glioma and other miR-185* high-expression tumors.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to the purposes of a kind of microRNAs (miRNA), especially relate to people's microRNA-185 * (human miR-185 * ) antisense nucleic acid and its application. The antisense nucleic acid can be combined with human miR-185 * Complementary, thereby inhibiting human miR-185 * expression to play an antitumor role. The invention also relates to a pharmaceutical composition containing the miRNA antisense nucleic acid. Background technique [0002] miRNAs are small non-coding RNAs, 20-25bp in length, usually transcribed by RNA polymerase II (Pol II), and generally the initial product is a large one with a cap structure (7MGpppG) and a polyA tail (AAAAA) The pri-miRNA. These pri-miRNAs are processed into pre-miRNA precursor products consisting of 70 nucleotides under the action of RNase III Drosha and its cofactor Pasha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P25/00
Inventor 丁侃张佩琢段春晓李捷沈孝坤
Owner SUZHOU GENEPHARMA
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