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Trichoderma pill as well as preparation method and application thereof

A technology for dropping pills and Trichoderma, which is applied in the field of Trichoderma dropping pills and their preparation, can solve the problems of unreasonable application, easy inactivation of microorganisms, etc., and achieve the effects of easy popularization, significant antagonistic effect, and good molding effect.

Inactive Publication Date: 2011-08-10
惠州市南天生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the drug loading of the drop pill preparation is closely related to the shape of the drop pill, and the processing temperature must also be strictly controlled to prevent microorganisms from easily losing their activity under high temperature conditions.
So far, drop pill technology has not been rationally applied in the field of microbial active pesticide preparation

Method used

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  • Trichoderma pill as well as preparation method and application thereof
  • Trichoderma pill as well as preparation method and application thereof
  • Trichoderma pill as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kind of Trichoderma drop pill, containing Trichoderma spore suspension, preservative and drop pill matrix, wherein, Trichoderma spore suspension is made of Trichoderma aculeatus strain T 1 (Trichoderma asperellum T 1 , preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC NO: 3531) prepared from spore powder, its rDNA ITS region fragment sequence is shown in SEQ ID NO: 1; the preservative is salicylic acid, the concentration The spore suspension is 1 g / L; the matrix is ​​a mixture of PET2000 and PET4000 with a mass ratio of 1:1, and the mass ratio of the original drug (ie Trichoderma spore suspension and salicylic acid) to the matrix is ​​1:10.

[0036] The preparation method of present embodiment trichoderma dropping pill is as follows:

[0037] 1. Recovery and expansion of strains

[0038] (1) The refrigerated Trichoderma aculeatus strain T 1(Trichoderma asperellum T 1 ) i...

Embodiment 2

[0047] Further illustrate below the impact of the change of parameters such as substrate, medicinal liquid content, medicinal liquid temperature on Trichoderma drop pill preparation.

[0048] Taking the difficulty of dripping and the effect of forming pills as indicators, a single factor analysis was performed on the choice of matrix of the dropping pills and the content of the liquid medicine. content and matrix on the prepared dripping pills, so as to compare and select the matrix and liquid content that are beneficial to ensure that the dripping pills are well formed and have certain biocontrol activity.

[0049] From the water-soluble dripping pill matrix polyethylene glycol, select the matrix with a low melting point and good pilling effect, set the ratio of four medicinal liquid matrixes such as 1:10, 2:10, 3:10, 4:10, etc., and measure each ratio Difficulty and pill formation of the prepared drop pills. The measured results are shown in Table 1 and Table 2:

[0050] T...

Embodiment 3

[0062] The L9 (3 3 ) Orthogonal test, with the formation rate of dripping pills and biological activity as the investigation index, select the appropriate preparation process. As shown in Table 3 and Table 4:

[0063] Table 3 Trichoderma drop pill formulation optimization orthogonal test factor level table

[0064]

[0065] According to the orthogonal test design, the working parameters of the dropping pill machine were set, and the liquid paraffin was used as the condensing agent to carry out the experiment. The different substrates were melted by the hot melt method and mixed with the Trichoderma spore liquid evenly, and added to the dropping pill machine for dripping, and 9 The Trichoderma dripping pills of a variety of preparation processes were tested and scored for each index. in The detection of weight difference and the inspection of dissolution time limit are carried out according to the method of "Chinese Pharmacopoeia", and the detection of active ingredient...

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Abstract

The invention belongs to the technical field of pesticides with microbial activity. A trichoderma pill provided by the invention comprises a trichoderma spore suspension and a pill matrix, wherein the trichoderma T1 bacterial strain spore suspension is prepared from spore powder of trichoderma asperellum T1 which is preserved in an ordinary microorganism center of the CCCCM (China Committee for Culture Collection of Microorganisms), wherein the preservation number is CGMCC (China General Microbiological Culture Collection) NO: 3531 and the preservation date is December 17, 2009; the fragment sequence in DNA ITS region is shown as SEQ ID NO:1; a preservative agent of a spore suspension with the concentration of 0.5-1.5g / l is contained in the invention; and the pill matrix is a mixture of one or two or three of polyethylene glycol 1000, polyethylene glycol 2000 and polyethylene glycol 4000. The trichoderma pill provided by the invention has the advantages of high dose content, high biological activity and good molding effect, can be dissolved in the shortest time, and is convenient for storage, transportation and use.

Description

technical field [0001] The invention belongs to the technical field of microbial active pesticides, and in particular relates to a trichoderma dropping pill and its preparation method and application. Background technique [0002] Trichoderma belongs to the subphylum Deuteromycetes, the order Conglomerate, and belongs to the family Stemaceae. It is a class of biocontrol and beneficial bacteria with important economic significance. Trichoderma has strong adaptability to the environment, grows much faster than pathogenic fungi, and is a strong competitor for the living space and nutrient source of plant pathogenic fungi in soil. Trichoderma can effectively use low-concentration nutrients on the plant surface or near the invasion point of plant pathogenic fungi to quickly occupy the space and absorb nutrients so that plant pathogenic fungi cannot invade. In recent years, the research on artificially introducing Trichoderma into the soil to control plant diseases has been activ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/34A01P3/00
Inventor 方羽生张颂声周俐钟平生詹玉海李小妮高永峰陈树华蒋刚彪李静美
Owner 惠州市南天生物科技有限公司
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