Small molecular non-coding RNA gene hsa-miR-29b and use thereof

A non-coding, small-molecule technology applied in the field of RNA and its applications

Inactive Publication Date: 2011-08-10
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on hsa-miR-29b and tumor angiogenesis in the existing domestic and foreign literatures.

Method used

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  • Small molecular non-coding RNA gene hsa-miR-29b and use thereof
  • Small molecular non-coding RNA gene hsa-miR-29b and use thereof
  • Small molecular non-coding RNA gene hsa-miR-29b and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: In Vitro Tube Formation Experiment

[0059] The in vitro tube formation experiment is an experiment in which vascular endothelial cells form a lumen structure in vivo by simulating in vitro. Angiogenesis involves the degradation of vascular basement membrane, the proliferation and migration of vascular endothelial cells into the extracellular matrix and the reorganization of vascular endothelial cells into a three-dimensional tubular structure to form a new basement membrane. In the in vitro tube formation experiment, human umbilical vein endothelial cells HUVEC were provided with extracellular matrix by laying matrigel to simulate the in vivo environment. Human umbilical vein endothelial cells HUVEC formed a two-dimensional tubular structure through processes such as proliferation, migration and adhesion. Budding is an essential step in angiogenesis. Therefore, in vitro, the budding ability of HUVEC can be evaluated by calculating the number of nodes of the ...

Embodiment 2

[0100] Embodiment 2 Tumor formation and immunohistochemical experiments in nude mice

[0101] 2.1 Cell transfection

[0102] Use Invitrogen’s transfection reagent Lipofectamine RNAi MAX to transfect the target RNA (hsa-miR-29b or NC) into the hepatocellular carcinoma cell line LM6 (LM6 for short, from Shanghai Cell Institute), the final concentration of the target RNA is 50nM, after 48 hours of culture Digested with trypsin and counted according to 1 × 10 7 The concentration of cells / ml was resuspended in PBS. Reagents and materials not indicated in specific sources in this example are commercially available standard samples.

[0103] 2.2 Tumor formation in nude mice

[0104] Five 5-week-old BALB / c nude mice were selected and subcutaneously injected with 1×10 6 Restoration of hsa-miR-29b or NC expression in LM6 tumor cells. Continuously observe and measure the size of the tumor for more than 1 month. The tumor tissues of all nude mice were completely stripped, and made i...

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PUM

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Abstract

The invention provides a small molecular non-coding RNA (named hsa-miR-29b) and use thereof. In the invention, in-vitro tube formation experiments and nude mouse tumorigenicity and immunohistochemistry experiments are performed, wherein the results of the in-vitro tube formation experiments indicate that the in-vitro restoration of the expression of the hsa-miR-29b can obviously inhibit angiogenesis induced by tumor cells; and the results of the nude mouse tumorigenicity and immunohistochemistry experiments indicate that the in-vivo restoration of the expression of the hsa-miR-29b can obviously inhibit the in-vivo tumorigenic capacity and angiogenic capacity of tumor cells and therefore can inhibit tumor hematogenous metastasis. Therefore, the hsa-miR-29b can be used for preparing medicines for inhibiting tumor cell induced angiogenesis, anti-tumor angiogenesis medicines, antitumor medicines and anti-tumor hematogenous metastasis medicines.

Description

technical field [0001] The present invention relates to an RNA and its application, in particular to a small molecule non-coding RNA and its application. Background technique [0002] Cancer (malignant tumor) has become a global public health problem, especially in our country. The Ministry of Health reported that the cancer death rate among urban and rural residents in China has increased by more than 80% in the past 30 years. At present, cancer has become the first cause of death of urban residents and the second cause of death of rural residents in China. Among them, primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the most common malignant tumors in the world, and its incidence rate and mortality rate are both in the forefront of various malignant tumors. China is a high-incidence area for liver cancer, accounting for 55% of the total number of HCC patients in the world. Radical resection is the main treatment method for malignant tumors toda...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00A61P35/04
Inventor 庄诗美方坚鸿周慧超
Owner SUN YAT SEN UNIV
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