Self-curling membrane agent applied to nerve repair and preparation method thereof

A self-curling film agent and self-curling film technology are applied in the fields of medical preparations containing active ingredients, pharmaceutical formulations, pharmaceutical sciences, etc., and can solve the problem of not being able to simultaneously meet the needs of rapid repair and treatment of nerve damage.

Active Publication Date: 2019-07-23
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the bottleneck that "existing preparations cannot simultaneously meet all the requirements for rapid repair of nerve damage", and provide a film agent with a fast self-crimping to form a lumen structure that meets the requirements for repairing defective nerves. The quick fix provides optimal conditions

Method used

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  • Self-curling membrane agent applied to nerve repair and preparation method thereof
  • Self-curling membrane agent applied to nerve repair and preparation method thereof
  • Self-curling membrane agent applied to nerve repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 The preparation of silk fibroin self-curling film agent

[0028] Preparation method: According to the design in Table 1, the experimental group of the self-rolling film was prepared according to the following scheme.

[0029] (1) Mix 0.5g of sodium alginate, 0.01g of cyclic dipeptide, 0.2ml of glycerin and 0.072g of disodium edetate in a beaker, add 24ml of distilled water, seal the beaker with a parafilm, and let it stand to make it fully Swell, then stir for 2 hours to dissolve evenly, and let stand at 4°C for 24 hours to remove air bubbles in the solution.

[0030] (2) Take out 10 mL of sodium alginate solution with a syringe, cast it on a mold with a surrounding edge to form a film, place it in a constant temperature oven at 60°C, and dry it to form a smooth and flat film.

[0031] (3) Prepare 2% calcium chloride solution, pour it on the membrane agent of (2), completely submerge the membrane agent, and cross-link for 30 seconds. The surface solution ...

Embodiment 2

[0037] The physical characteristic of embodiment 2 self-curling film agent

[0038] The self-curling film agent experimental group and the control group of embodiment 1 spray physiological saline under 785nm infrared light irradiation, observe the tube-forming ability of the self-curling film agent to form a closed lumen, record the tube-forming time, and measure the elasticity of the closed tube respectively Modulus, the results are shown in Table 2.

[0039] Table 2 The physical characteristics of the self-rolling film experimental group and the control group

[0040]

Embodiment 3

[0041] Embodiment 3 The effect of self-curling film agent applied to nerve repair in vivo

[0042] (1) Rat sciatic nerve defect model

[0043] Animal preparation: healthy adult male SD rats, weighing 220-250 g, free to eat and drink, and adaptively fed for 1 week.

[0044] Modeling surgery: Animals were fasted for 12 hours before surgery. After abdominal anesthesia with 10% chloral hydrate (0.3mL / 100g), the hair on the right leg was shaved with a small animal shaver, and the animal was fixed on the experimental animal operating table in a prone position. After iodophor disinfected the surgical area of ​​the right leg, it was wiped again with alcohol, and the skin was cut parallel to the femur in the rat's right buttock area with surgical scissors. After the skin is cut, a solid and movable sheath of subcutaneous connective tissue can be seen covering the sarcolemma. Use blunt scissors to open the sarcolemma, and dissect the biceps femoris covered by the sarcolemma in a blunt...

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Abstract

The invention provides a self-curling membrane agent applied to nerve repair and a preparation method thereof. The self-curling membrane agent is a membrane agent which is composed of three layers ofA, B and C and has the thickness of 60-120 [mu]m. The main components of the A layer are indocyanine green and silk fibroin, the main components of the B layer comprise cyclic dipeptide and calcium alginate, and the main components of the C layer comprise cyclic dipeptide and sodium alginate. The membrane agent directionally curls in water to form a continuous hollow tube, the A layer is arrangedinside, the C layer is arranged outside, and the membrane agent quickly becomes a closed lumen structure under the irradiation of near red light. The invention provides a new method for nerve dockingand casing repair in nerve defects, realizes the requirements of simple and convenient manufacture, convenient tube formation, controllable size and convenient application, and has wide application prospect.

Description

technical field [0001] The invention relates to a film for nerve repair treatment, in particular to the composition, preparation and application of a self-rolling film capable of maintaining a favorable microenvironment at a nerve injury site and ensuring the nerve repair process for a long time. Background technique [0002] Today, global terrorist attacks and natural disasters are becoming more and more frequent. According to incomplete statistics, there are about 1 million new cases of peripheral nerve injury in China every year, including about 300,000 cases of nerve defect. In clinical work, long-segment nerve defect caused by Nerve injury often has a poor prognosis and postoperative functional recovery of patients is poor, which brings a heavy burden to the society, family and patients themselves. In existing studies, nerve conduits are often used as a scaffold material combined with cells or growth factors to repair nerve damage. However, the disadvantages of poor tub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/22A61L27/54A61K9/00A61K41/00A61P15/00
CPCA61K9/0034A61K41/0023A61L27/20A61L27/227A61L27/54A61L2300/412A61L2430/32A61P15/00C08L5/04
Inventor 赵应征鲁翠涛虞希冲金冰慧林蒙婷王丽芬黄志伟
Owner WENZHOU MEDICAL UNIV
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