Method for detecting safety of insect-resistant transgenic corn on non-target organisms

An insect-resistant gene and safe technology, which is applied in the field of detecting whether transgenic insect-resistant corn is safe to non-target organisms, can solve the problems of impossible non-target biological safety, and achieve convenient, fast and accurate investigation and counting. The effect of judgment

Inactive Publication Date: 2011-08-17
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For safety testing of genetically modified organisms, currently only some dominant species or economically important species such as si

Method used

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  • Method for detecting safety of insect-resistant transgenic corn on non-target organisms
  • Method for detecting safety of insect-resistant transgenic corn on non-target organisms
  • Method for detecting safety of insect-resistant transgenic corn on non-target organisms

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0063] Example 1. Detection of whether transgenic Bt corn is safe for non-target organisms

[0064] In 2009 and 2010, leafhoppers were used in the genetically modified crop test base of China Agricultural University in Beijing to carry out research on the impact of transgenic Cry1Ac maize on non-target organisms.

[0065] 1. Method

[0066] 1. Material selection

[0067] 2009: (1) Transgenic corn group (denoted as transgenic); (2) Parental control group (denoted as parent).

[0068] 2010: (1) Transgenic corn group (denoted as transgenic); (2) Parental control group (denoted as parent); (3) Conventional hybrid varieties (denoted as Xianyu).

[0069] 2. Location selection

[0070] The test site is more than 500 meters away from other corn fields, and the fertility conditions of the test plots are basically the same. Each plot is about 25×30 meters, divided into 15 plots, with 5 repeats of each variety. The area of ​​each plot is set at 45m 2 (5m×9m), 15 rows are planted in each plot, 20 p...

Example Embodiment

[0157] Example 2. The effect of transgenic corn on the growth and development of Harmonia axyridis

[0158] Toxicological experiment: every 30 newly hatched ladybug larvae constitute a group, a total of 8 groups. Among them, 4 groups were fed with Bt transgenic corn pollen and aphids at a 2:1 ratio (weight ratio) as a treatment; 4 groups were fed with non-transgenic Bt corn pollen and aphids at a 2:1 ratio as a control. The ladybird larvae were reared in a finger tube with one head, and their growth and development were observed every day, and the development period of the ladybirds at different instars was recorded. Weighed every other day from the larva to the pre-adult stage, and in the adult stage (45 days, Counting from the 1st instar) the last time to evaluate the effect of transgenic insect-resistant corn pollen on ladybirds.

[0159] The result is Picture 9 Shown. The results showed that genetically modified corn is not toxic to Harmonia axyridis.

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Abstract

The invention discloses a method for detecting safety of insect-resistant transgenic corn on non-target organisms. The method for detecting the safety of the insect-resistant transgenic corn on the non-target organisms comprises the following steps of: selecting insects with sucking mouth parts in the non-target organisms as a population to be detected, detecting whether an insect-resistant transgenic plant is safe for the population to be detected, if so, determining that the insect-resistant transgenic plant is safe for the non-target organisms in candidate, otherwise, and determining that the insect-resistant transgenic plant is not safe for the non-target organisms in candidate. The method makes up the defect that the insects with chewing mouth parts are separately used as research objects in the field of influence research of the transgenic corn on the non-target organisms, has significance for completing the influence research of transgenic crops on the non-target organisms, and has significance for determining the biological safety of the transgenic plants.

Description

technical field [0001] The invention relates to a method for detecting whether the transgenic insect-resistant gene corn is safe to non-target organisms. Background technique [0002] The main problem with traditional chemical insecticides is that they are toxic to other insects, animals and even humans who are not harmful. While killing target pests, pesticides also kill non-target insects, such as secondary pests, neutral insects, important economic insects, natural enemies of pests, and other non-target organisms, such as soil microorganisms, thereby disrupting the ecological balance. The application of insect-resistant transgenic crops is an important way to replace insecticides. [0003] Insect-resistant transgenic crops may also have potential negative effects, such as pest resistance to insect-resistant genes and impact on non-target organisms. It is of great significance for the healthy development of transgenic technology to conduct in-depth research on the potent...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 石旺鹏赵可董学会郭艳艳
Owner CHINA AGRI UNIV
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