In-situ collection device for plant root secretions and application thereof

Abstract

The invention belongs to the technical field of soil and plant science, particularly relates to an in-situ collection device for plant root secretions and application thereof, wherein a movable drawer is arranged at the lower layer of a connection box, so that the collection box is separated into two layers, i.e., an upper layer and a lower layer; small holes are fully distributed on the bottom of the upper layer of the connection box; and a layer of fine gauze is covered on the bottom of the upper layer of the connection box. Soil is used as the base material of the upper layer of the connection box; the lower drawer is provided with deionized water or other aqueous solution; and the roots of the plants planted in the upper layer enter the lower drawer through the bottom where small holes are fully distributed. The invention provides a novel in-situ collection device for quantitatively researching the species of the root secretions, so that the in-situ collection device has a significant application value for researching the root secretion conditions of original ecological plants.

Description

technical field [0001] The invention belongs to the field of soil and plant science and technology, and in particular relates to a device for collecting plant root exudates in situ and its application. Background technique [0002] In the study of root exudates, the research methods of collection, separation and determination of root exudates have always been one of the frontiers and difficulties in this research field. Root exudates come in a wide variety and vary greatly in quantity, including primary metabolites such as sugar, protein, and amino acids, and secondary metabolites such as organic acids and phenols. Root exudates vary greatly due to plant species, growth stages and abiotic conditions, and are also related to microbial degradation and root damage. If collected in soil, it is related to the location of the micro sampler. Since there are many factors affecting root exudates, it is particularly important to choose scientific research devices and methods in order...

AI Technical Summary

Problems solved by technology

Among them, the amount of root exudates collected under sterilized conditions can accurately reflect the content of organic and inorganic substances secreted by plant roots, but the types and contents of these measured root exudates and plant growth in the soil are quite different , cannot reflect the real state; root exudates collected in situ under traditional soil culture conditions can directly reflect the actual situation of root exudates, but there are also problems such as inaccurate quantification, soil adsorption and microbial degradation

[0004] Currently, we are faced with the following three problems when studying plant root exudates: (1) the exudates to be collected are usually of extremely low content, requiring a large number of plants and prolonged collection time to obtain a sufficient amount of exudates; (2) ) The collection and processing of samples is cumbersome, and some unstable compounds will be denatured; (3) The growth substrate, container, nutrient solution, extraction agent, etc. will cause serious pollution to the sample when the traditional solvent extraction method collects trace secretions

The current in-situ collection method is the soil solution sampler method, which can directly extract the soil solution in situ, but its PVC pipe contains 30% plasticizer and a small amount of stabilizer, and these residual substances can enter the sample. In addition, The solution taken often contains a large amount of soil organic matter, so it will also cause greater pollution and interference to the sample

In addition, soil is a very complex multiphase system, the factors affecting soil solution chemistry are not yet clear, and there are spatiotemporal variations in soil and microbial degradation of root exudates, making it more difficult to collect solutions that represent true root exudates

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