Physiological parameter-based fast identification method of resistance of wheat scab

Abstract

The invention discloses a physiological parameter based fast identification method of the resistance of wheat scab, comprising the following steps of: (1) planting materials with different genotypes; (2) carrying out wheat scab inoculation by adopting a spore coating or single flower instillation method; (3) recording illness states: respectively surveying a pathogenic spikelet number and a total spikelet number at seven, fourteen, twenty-one and twenty-eight days after the wheat scab inoculation, and calculating pathogenic spikelet rate; measuring Net photosynthesis rate (Pn), stomatic conductance (Gs) and intercellular CO2 concentration (Ci) by utilizing an Li-6400 photosynthetic measurement system at seven and twenty-one days after the wheat scab inoculation, wherein temperature is 21-24 DEG C, saturated vapour pressure difference is 0.55-0.65 kPa, illumination intensity is 1000 mmol m<-2> s<-1>, and measuring time is 30 seconds at a time; and (4) carrying out statistic analysis to obtain an identification conclusion: carrying out the statistic analysis to obtain the identification conclusion by utilizing SPSS (Statistical Package for the Social Science) software.

Description

technical field [0001] The invention relates to a rapid identification method for wheat scab resistance based on physiological parameters. Background technique [0002] Scab is a worldwide wheat disease, which not only reduces the yield, but also affects the commercial quality of wheat and harms the health of humans and animals. In recent decades, wheat breeding for scab resistance has received widespread attention worldwide, and a lot of manpower and material resources have been invested. Liu Dajun (2001) pointed out that the genetics and breeding of wheat scab resistance is a worldwide problem, and in order to achieve new breakthroughs, new ideas are needed for scientific research on scab. [0003] In the past, some researchers used the same genus materials to identify head blight resistance, and got the opposite results. The main reason for this contradiction was that they did not pay attention to the intraspecific variability of wheat exogenous species, which was not ba...

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Problems solved by technology

[0014] The accuracy and repeatability of the evaluation methods currently used are poor, because the identification methods of resistance inoculation in the past mainly relied on the naked eye identification of research workers, and different people have different identification abilities, and the disease performance of different wheat materials also varies. Certain differences make the existing resistance identification methods not necessarily artificial and arbitrary

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