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Preparation method and application of time temperature indicator based on lipase reaction diffusion

A time temperature, indicator technology, applied in thermometers that give average/integrated values, material analysis by observing the effect on chemical indicators, and analysis by causing chemical reactions to occur in materials, etc., can solve the color ring. The color band becomes lighter, the diffusion is difficult, the identification is difficult, etc., to achieve the effect of complete gel structure, obvious diffusion change, and obvious diffusion phenomenon.

Active Publication Date: 2011-09-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This TTI combines enzyme chemical reaction and diffusion principle very well, but there will be the following problems in practical application: (1) The change of indicator color circle is not obvious enough, which is manifested in: the diffusion area is small, and the maximum radius is 7- 8mm; as the diffusion distance increases, the color band of the color ring becomes lighter; the boundary of the color ring is blurred during the diffusion process, and the diffusion area is small, making it difficult to identify and the indication effect is not ideal
(2) The enzyme in TTI has not undergone any treatment and is easily inactivated, so it cannot be used for the prediction of long shelf life foods
In addition, enzymes are biological macromolecules, and it is very difficult to diffuse into agar plates with poor permeability
(3) The enzyme solution used in TTI is liquid, which is inconvenient for practical application and inconvenient for activation, resulting in a more complex structure of the designed TTI, high cost, and insufficient stability of the effect

Method used

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  • Preparation method and application of time temperature indicator based on lipase reaction diffusion
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  • Preparation method and application of time temperature indicator based on lipase reaction diffusion

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Effect test

Embodiment 1

[0048] Embodiment 1: The preparation method of the time temperature indicator based on lipase reaction and diffusion principle of the present invention adopts the following preparation steps:

[0049] 1, the preparation of the gel-like reaction substrate: add 0.5mL triacetin (reaction substrate) in 50ml small beaker, then be 8% polyvinyl alcohol (polymerization degree 1750 ± 50) Aqueous solution, set the volume to 10mL (volume ratio of glycerol triacetate (reaction substrate) to polyvinyl alcohol aqueous solution is 5:95), stir with a stirrer at a speed of 180-220r / min for 2min to obtain a uniform emulsion, Take 0.3mL and pour it into the gel substrate groove of the upper cover 1, place it horizontally in the refrigerator, and freeze it at -20°C for 16 hours, then take it out, and thaw it at room temperature for 6 hours to obtain a square gel-like reaction substrate 2, The size is 10mm×10mm×3mm.

[0050] 2. Preparation of the enzyme substrate: Add 1mL of the prepared Gly-NaOH...

Embodiment 2

[0059] Embodiment 2: The preparation method of the time temperature indicator based on lipase reaction and diffusion principle of the present invention adopts the following preparation steps:

[0060] 1, the preparation of the gel-like reaction substrate: add 1.5mL triacetin (reaction substrate) in 50ml small beaker, then be 8% polyvinyl alcohol (polymerization degree 1750 ± 50) Aqueous solution, set the volume to 10mL (the volume ratio of glycerol triacetate (reaction substrate) to polyvinyl alcohol aqueous solution is 15:85), stir with a stirrer at a speed of 180-220r / min for 2min to obtain a uniform emulsion, Take 0.3mL and pour it into the gel substrate groove of the upper cover 1, place it horizontally in the refrigerator, and freeze it at -20°C for 16 hours, then take it out, and thaw it at room temperature for 6 hours to obtain a square gel-like reaction substrate 2, The size is 10mm×10mm×3mm.

[0061] 2. Preparation of the enzyme substrate: Add 2 mL of prepared Gly-Na...

Embodiment 3

[0067] Embodiment 3: The preparation method of the time temperature indicator based on lipase reaction and diffusion principle of the present invention adopts the following preparation steps:

[0068] 1. Preparation of gel-like reaction substrate: add 1.2mL triacetin (reaction substrate) in a 50ml small beaker, then use an aqueous solution of polyvinyl alcohol (polymerization degree 1750 ± 50) with a mass concentration of 8%, Set the volume to 10mL (the volume ratio of glycerol triacetate (reaction substrate) to polyvinyl alcohol aqueous solution is 12:88), stir with a mixer at a speed of 180-220r / min for 2min to obtain a uniform emulsion, take 0.3mL and inject Put it into the gel substrate groove of the upper cover 1, place it horizontally in the refrigerator, and freeze it at -20°C for 16 hours, then take it out, and thaw it at room temperature for 6 hours to obtain a square gel-like reaction substrate 2 with a size of 10mm× 10mm×3mm.

[0069] 2. Preparation of enzyme subst...

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Abstract

The invention relates to a preparation method and application of a time temperature indicator based on lipase reaction diffusion. The preparation method comprises the following steps of: preparing an upper cover containing a gel reaction substrate and a bottom board containing a gel enzyme substrate; before activation, separating the upper cover containing the gel reaction substrate from the bottom board containing the gel enzyme substrate and refrigerating at the temperature of 4 DEG C; during use, pressing the upper cover containing the gel reaction substrate on the bottom board containing the gel enzyme substrate, so that substrate gel of the gel reaction substrate is contacted with the gel enzyme substrate for activation; performing an enzymatic reaction after the substrate gel is contacted with the gel enzyme substrate to form a yellow belt; judging and determining the accumulation effect of the yellow belt on time and temperatures according to the diffusion length of the yellow belt; and performing the matching of activation energy with food to be predicted according to the relation between the activation energy of an established time temperature indicator (TTI) and influence factors, so that the quality condition of the food during production, storage and transportation and sale can be monitored conveniently and reliably.

Description

technical field [0001] The invention relates to a time-temperature indicator based on the principle of lipase reaction and diffusion and a method for using it. The time-temperature indicator is based on the principle of lipase reaction and diffusion, and can indicate the time and temperature of an item in the process of production, storage, transportation and sales. Accumulation effect, so that it is convenient and reliable to monitor the quality status of items. Background technique [0002] With the improvement of people's living standards, food safety issues are getting more and more attention from consumers. The issue of shelf life is an important aspect of food safety, and the influence of temperature on shelf life is crucial, especially for those foods that are greatly affected by temperature (such as some easily spoiled foods that need to be frozen or refrigerated). The shelf life (that is, the open shelf life) on the outer label of the package that people usually se...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01K3/04
Inventor 卢立新蔡莹郑伟洲
Owner JIANGNAN UNIV
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