Preparation method of enzyme-hydrolyzed butter with high flavor intensity and use thereof in essence with milk flavor
An enzymatic hydrolysis and butter technology, which is applied in the field of food additives, can solve the problems such as the inability to meet the requirements of the concentration of essence, and achieve the effects of obvious flavor enhancement, high aroma intensity and short enzymatic hydrolysis time.
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Embodiment 1
[0017] A method for preparing enzymatically hydrolyzed butter with high flavor intensity, using 0.15 part of lipase AY30 and 0.15 part of lipase M10. Specific steps are as follows:
[0018] 1) Separation and screening of lipase, using a neutral system prepared by mixing butter and water as a screening model. Put the New Zealand anhydrous butter into the reaction tank, heat and melt, and control the temperature at about 50°C. Prepare a buffer solution with 0.8 parts of disodium hydrogen phosphate, 0.2 parts of potassium dihydrogen phosphate and 24 parts of sterile water, and put it into the reaction tank. The purpose of configuring the buffer solution is to make the change of the pH value of the enzymolysis system less drastic and to prolong the effective action time of the enzyme.
[0019] 2) Carry out enzymolysis reaction with the screened enzymes to obtain a group of corresponding enzymolysis butter products; the process conditions of the enzymolysis reaction are as follow...
Embodiment 2
[0021] Yet another method for preparing enzymatically hydrolyzed butter with high flavor intensity, using 0.12 parts of Lipozyme TL 100L lipase and 0.12 parts of Novozym 435 lipase.
[0022] Put the New Zealand anhydrous butter into the reaction tank, heat and melt, and control the temperature at about 50°C. Prepare a buffer solution with 0.8 parts of disodium hydrogen phosphate, 0.2 parts of potassium dihydrogen phosphate and 24 parts of sterile water, and put it into the reaction tank. The purpose of configuring the buffer solution is to make the change of the pH value of the enzymolysis system less drastic and to prolong the effective action time of the enzyme. Continue to raise the temperature of the material in the reaction tank to 85° C., and maintain this temperature for 15 minutes to carry out sterilization treatment before enzymatic hydrolysis. The material temperature in the reaction tank was cooled to 40° C. and maintained at this temperature. Dissolve 0.12 parts ...
Embodiment 3
[0024] Another method for preparing enzymatically hydrolyzed butter with high flavor intensity uses 0.12 part of Japanese name sugar lipase.
[0025] Put the New Zealand anhydrous butter into the reaction tank, heat and melt, and control the temperature at about 50°C. Prepare a buffer solution with 0.8 parts of disodium hydrogen phosphate, 0.2 parts of potassium dihydrogen phosphate and 24 parts of sterile water, and put it into the reaction tank. The purpose of configuring the buffer solution is to make the change of the pH value of the enzymolysis system less drastic and to prolong the effective action time of the enzyme. Continue to raise the temperature of the material in the reaction tank to 85° C., and maintain this temperature for 15 minutes to carry out sterilization treatment before enzymatic hydrolysis. The material temperature in the reaction tank was cooled to 40° C. and maintained at this temperature. Dissolve 0.12 parts of Japanese name sugar lipase in steriliz...
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