Culture method and applications of Pseudomonas aurantiaca

A technology of Pseudomonas orange and a cultivation method, which is applied to the cultivation method and application field of Pseudomonas orange, can solve the problems of destroying ecological balance, environmental pollution, slow reproduction and the like, and achieves growth promotion and easy use. , the effect of easy fermentation and reproduction

Inactive Publication Date: 2011-09-28
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology

The disadvantages of existing chemical control are: long-term use destroys ecological balance, causes secondary diseases to be rampant, and chemical residues cause environmental pollution, etc.
The shortcoming of existing Pseudomonas citricum is: Pseudomonas citricum exists in a sma

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  • Culture method and applications of Pseudomonas aurantiaca
  • Culture method and applications of Pseudomonas aurantiaca
  • Culture method and applications of Pseudomonas aurantiaca

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Embodiment Construction

[0065] The present invention will be further described below through specific embodiments.

[0066] Screening of bacterial strains: put the soil samples for testing into 1 / 3KB medium containing 40 μg / mL ampicillin and 13 μg / mL chloramphenicol, and cultivate for 6 hours. The enriched bacterial solution was plated on the KB solid medium containing 40 μg / mL ampicillin and 13 μg / mL chloramphenicol, plated according to gradient dilution, and 276 strains were obtained by separation and purification, which were used as preliminary screening for future use. Sclerotinia sclerotiorum was used as indicator bacteria, and the isolated bacterial strains were screened for antagonistic properties by plate confrontation growth method. The center of the PDA plate with an inner diameter of 90 mm was inoculated with a cake of Sclerotinia sclerotiorum with a diameter of 8 mm, and four isolated and purified bacterial strains to be tested were spotted at the four corners of the cake at a distance of...

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Abstract

The invention belongs to the biological technology and relates to a culture method and applications of Pseudomonas aurantiaca. The prior art shows that Pseudomonas aurantiaca can inhibit various plant pathogenic bacteria; but a small amount of Pseudomonas aurantiaca exists in nature, the propagation speed of Pseudomonas aurantiaca is low and the demands on the disease control of plant and the growth improvement of plant can not be met. The culture method of Pseudomonas aurantiaca comprises the following steps: collecting a sample; preparing a culture medium; selecting and determining the antibiotic and tested pathogenic fungus; performing primary screening and secondary screening of the strain; and using the finally screened strain to perform fermentation and propagation. The obtained Pseudomonas aurantiaca can be utilized to control the diseases of rape, rice, wheat, tomato and corn. The invention has the following advantages: the obtained Pseudomonas aurantiaca has efficient antibacterial activity and remarkable control effect of plant diseases; and the Pseudomonas aurantiaca is safe and non-toxic for people and livestock, and can be used for promoting the growth of crop and be easy to propagate and convenient to use.

Description

technical field [0001] The invention belongs to biological technology, in particular to a cultivation method and application of Pseudomonas orange. Background technique [0002] Plant diseases cause crop quality deterioration and yield decline, bringing huge economic losses and safety hazards to agricultural production and food safety. The prevention and control method of plant disease in the prior art is mainly to utilize the chemical control of pesticide. Chemical agents have the advantages of fast control speed, good effect, wide insecticidal and antibacterial spectrum, low cost, and simple use. The disadvantages of the existing chemical control are: long-term use destroys the ecological balance, causes rampant secondary diseases, and causes environmental pollution due to chemical residues. [0003] The biological control of plant diseases is a method of using harmless or beneficial organisms to affect or inhibit the survival and activity of pathogens, reduce the occurr...

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/38
Inventor 朱再玲王慧捷肖明
Owner SHANGHAI NORMAL UNIVERSITY
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