Nanotherapeutic colloidal metal compositions and methods

A technology of colloidal metals and compositions, applied in the direction of hybrid methods, nano-medicines, chemical instruments and methods, etc.

Inactive Publication Date: 2011-09-28
CYTIMMUNE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such treatments provide treatment of the condition, but also require a number of adjunctive treatments to treat side effects

Method used

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  • Nanotherapeutic colloidal metal compositions and methods
  • Nanotherapeutic colloidal metal compositions and methods
  • Nanotherapeutic colloidal metal compositions and methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0162] Preparation of colloidal gold sol

[0163] Chlorauric acid (Au +3 ;HAuCl 4 ) reduced to neutral gold (Au 0 ) to prepare colloidal gold. The method described by Horisberger (1979) is suitable for the preparation of 34 nm colloidal gold particles. This method provides a simple and scalable procedure for preparing colloidal gold. In short, in deionized H 2 O(DIH 2 4% gold chloride solution (23.03% stock solution, dmc 2 , South Plainfield, NJ) and 1% sodium citrate solution (wt / wt; J.T. Baker Company; Paris, KY). Add 3.75 ml of gold chloride solution to 1.5 L of DIH 2 O middle. The solution was stirred vigorously and brought to a boil at reflux. Formation of 34 nm colloidal gold particles was initiated by adding 60 ml of sodium citrate. The solution was continuously boiled and stirred during the entire process of particle formation and growth as described below.

[0164] The addition of sodium citrate to gold chloride initiates a series of reduction reactions ch...

Embodiment 2

[0169] metal source

[0170] Experiments were performed to see if the source of the starting gold reactants that formed the colloidal structure affected the composition of the colloidal gold. Gold chloride was purchased from two different commercial sources: Degussa Metals Catalysts Cerdec (dmc 2 ) and Sigma Chemical Company. The two gold preparations were analyzed for the presence of contaminating metals and other substances. The results of these studies are listed in Table II. Although the gold concentration in each formulation was within the reported values, it is clear that the Sigma formulation contained higher levels of Mg, Ca and Fe.

[0171] Table II. Purity of gold chloride salts used to produce colloidal gold

[0172] element

dmc 2

Sigma

Na

<25ppm

>21ppm

Mg

<25ppm

>60ppm

[0173] Ca

<25ppm

>60ppm

Fe

<25ppm

>60ppm

[0174] TEM of the particles further showed that the...

Embodiment 3

[0177] Using Sigma and dmc 2 Preparation of colloidal gold sol by gold chloride

[0178] The above data indicate that from dmc 2 Gold Chloride contains lower levels of polluting elements. To determine the effect of these two qualitatively different sources of gold chloride, colloidal gold sols were prepared using the two different sources of the salt. The method used to create the colloidal gold particles was as originally described by Horisberger and in Example 1. In short, using from dmc 2 A 4% solution of gold chloride (in water) was prepared with the Sigma stock formulation. 3.75 ml of each solution was added to separate flasks each containing 1.5 L of water. The solution was brought to a rolling boil and kept boiling at reflux with vigorous stirring. 22.5 ml of a 1% sodium citrate solution was added to each flask. The solutions in both flasks were kept boiling until the already described process of colloidal gold formation was complete, marked by a change in gold c...

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Abstract

The present invention comprises compositions and methods for delivery systems of agents, including therapeutic compounds, pharmaceutical agents, drugs, detection agents, nucleic acid sequences and biological factors. In general, the nanotherapeutic compositions of the present invention comprise a platform comprising a colloidal metal, a targeting ligand such a tumor necrosis factor, a stealth agent such as polyethylene glycol, and one or more diagnostic or therapeutic agents for delivery. The invention also comprises methods and compositions for making such nanotherapeutic compositions and for the treatment of cancer.

Description

[0001] Related Application Cross Reference [0002] This application claims U.S. Provisional Patent Application No. 60 / 974,310 filed September 21, 2007, U.S. Provisional Patent Application No. 60 / 981,920 filed October 23, 2007, U.S. Provisional Patent Application No. 60 / 981,920 filed March 12, 2008 Application No. 61 / 069,108, U.S. Provisional Patent Application No. 61 / 069,905 filed March 19, 2008, U.S. Provisional Patent Application No. 61 / 040,022 filed March 27, 2008, April 11, 2008 U.S. Provisional Patent Application No. 61 / 123,796, U.S. Provisional Patent Application No. 61 / 124,290, filed April 15, 2008, and U.S. Provisional Patent Application No. 61 / 126,899, filed May 8, 2008. field of invention [0003] The present invention relates to compositions and methods for systemic delivery of agents and delivery of agents to specific sites. Generally, the present invention relates to colloidal metal compositions and methods for making and using such compositions. Background of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B82B1/00B01F3/12
CPCA61K47/48861B82Y5/00A61K47/6923A61P19/02A61P29/00A61P31/00A61P35/00A61P37/00A61P43/00
Inventor L·塔马金G·F·佩乔蒂M·S·胡塔
Owner CYTIMMUNE SCI
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