Method for producing vaccine by virtue of culturing animal cells

Abstract

The invention belongs to the field of vaccine preparation, and provides a method for producing a vaccine by virtue of culturing animal cells in a flake carrier. The method comprises the following steps: culturing vaccine stromal cells on the flake carrier; inoculating virus based on a certain concentration; continuously culturing cells and virus under a bioreactor control condition to obtain a virus solution; repeatedly freezing and thawing the flake virus and infection cells; and mixing all collected solutions to synthesize a virus suspension so as to prepare the vaccine. In the method, a flake carrier culturing technology is applied to producing the vaccine by virtue of culturing the animal cells in the bioreactor, the flake carrier provides a three-dimensional space and a larger specific surface area for cell growth and virus breeding, the cell density of the cells is higher, and the bioreactor controls culture conditions and other parameters to provide the best producing environment for cell growth and virus breeding, so that the virus titer is greatly reduced, and the vaccine yield and quality are more stable.

Description

technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a method for cultivating animal cells to produce vaccines, more specifically to a method for cultivating animal stromal cells to produce vaccines through sheet-like carriers. Background technique [0002] Traditional vaccine production technology often isolates and prepares virus liquid by inoculating chicken embryos or animals. However, due to individual and species differences, it is difficult for many viruses to find suitable sensitive animals, and the results are affected due to large individual differences. determination. With the development of cell culture technology, most viruses can have corresponding sensitive cells, which creates conditions for the proliferation of viruses. With the development of large-scale cell culture technology, as long as there are sensitive cells, large-scale Vaccine production. [0003] At present, the production of vaccines is mainly...

AI Technical Summary

Problems solved by technology

[0003] At present, the production of vaccines is mainly carried out through the spinning bottle process, but this method has a low degree of automation and high labor intensity, and because of the uncontrollable environment of the spinning bottle cell culture, the quality of the cells and vaccines is unstable, and the product batches vary greatly; On the other hand, the cell adsorption growth area provided by a single spinner bottle is limited, and the cell density can only reach 1-5×10 5 cells / ml, the production capacity is low, and at the same time, the only way to expand the production scale is to increase the number of bottles, resulting in greater demand for workshop scale, equipment access, personnel, etc., and greater labor.