Method for producing vaccine by virtue of culturing animal cells
A technology of animal cells and cultured cells, which is applied in the field of bioengineering and can solve problems such as low degree of automation, large differences in product batches, unstable quality of cells and vaccines, etc.
Inactive Publication Date: 2011-10-05
HANGZHOU AMPROTEIN BIOENG +1
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Abstract
The invention belongs to the field of vaccine preparation, and provides a method for producing a vaccine by virtue of culturing animal cells in a flake carrier. The method comprises the following steps: culturing vaccine stromal cells on the flake carrier; inoculating virus based on a certain concentration; continuously culturing cells and virus under a bioreactor control condition to obtain a virus solution; repeatedly freezing and thawing the flake virus and infection cells; and mixing all collected solutions to synthesize a virus suspension so as to prepare the vaccine. In the method, a flake carrier culturing technology is applied to producing the vaccine by virtue of culturing the animal cells in the bioreactor, the flake carrier provides a three-dimensional space and a larger specific surface area for cell growth and virus breeding, the cell density of the cells is higher, and the bioreactor controls culture conditions and other parameters to provide the best producing environment for cell growth and virus breeding, so that the virus titer is greatly reduced, and the vaccine yield and quality are more stable.
Application Domain
Vertebrate cellsArtificial cell constructs +2
Technology Topic
Virus suspensionTiter +10
Examples
- Experimental program(5)
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