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O.jingdongensis sp. antibacterial peptide and preparation method and use thereof

An antibacterial peptide, smelly frog technology, applied in the fields of biochemistry and biomedicine, can solve the problems of rising morbidity and mortality, and increasing medical expenses.

Inactive Publication Date: 2013-03-13
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the abuse and irrational use of antibiotics in the past 30 years, the widespread spread of drug-resistant strains has made anti-infection treatment a difficult problem, which will inevitably lead to an increase in morbidity and mortality and a substantial increase in medical expenses. Therefore, pathogen resistance to antibiotics is one of the most serious threats to global public health in the 21st century

Method used

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  • O.jingdongensis sp. antibacterial peptide and preparation method and use thereof
  • O.jingdongensis sp. antibacterial peptide and preparation method and use thereof
  • O.jingdongensis sp. antibacterial peptide and preparation method and use thereof

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Embodiment Construction

[0035]The specific embodiments will help those of ordinary skill in the art to further understand the present invention, but do not limit the scope of the present invention in any form. The experimental methods that do not indicate the specific conditions in the implementation methods are usually based on conventional conditions, such as in "Molecular Cloning Experiment Guide" (J. Sambrook, etc.) and "Refined Molecular Biology Experiment Guide" (F.M. Osper, etc. described conditions, or in accordance with the conditions recommended by the instrument manufacturer.

[0036] Preparation of Jingdong stink frog antimicrobial peptide Brevinin-1JDa:

[0037] The acquisition of the crude extract: Peel and dry the Jingdong stink frog, cut the dried skin into pieces, immerse in ethanol / 0.7M hydrochloric acid (3:1v / v) solution, stir in a magnetic stirrer for 3h, and rotate the extract solution to evaporate (60 ℃, 8min) after removing hydrochloric acid and most of ethanol, replenish wate...

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Abstract

The invention belongs to the field of biochemical and biomedicines and relates to an O.jingdongensis sp. antibacterial peptide and a preparation method and use thereof. The O.jingdongensis sp. antibacterial peptide is a single-chain polypeptide consisting of 24 amino acids, the theoretical monoisotopic molecular weight [M+H]+(monoisotopic mass) is 2600.5183Da, and the full-sequence structure of the O.jingdongensis sp. antibacterial peptide is NH2-FLPAVLRVAAKVVPTVFCLISKKC-COOH, wherein the two cysteines at a position-18 and a position-24, namely 18Cys and 24Cys, are in an oxidized disulfide bond (-S-S-) form. The O.jingdongensis sp. antibacterial peptide disclosed by the invention can be used for manufacturing antibacterial medicines or antibacterial agents.

Description

technical field [0001] The invention belongs to the fields of biochemistry and biomedicine, and relates to a Jingdong stink frog antibacterial peptide, its preparation method and application. Background technique [0002] The discovery of antibiotics is the most brilliant achievement of modern medicine in the 20th century. Thanks to the clinical application of antibiotics, many infectious diseases that were once considered fatal have been cured, thereby significantly increasing human life expectancy. However, due to the abuse and irrational use of antibiotics in the past 30 years, the widespread spread of drug-resistant strains has made anti-infection treatment a difficult problem, which will inevitably lead to an increase in morbidity and mortality and a substantial increase in medical expenses. Therefore, pathogen resistance to antibiotics is one of the most serious threats to global public health in the 21st century. On the one hand, the solution is to rationally use an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/20C07K1/18C07K1/16A61K38/17A61P31/04
CPCY02A50/30
Inventor 刘炯宇江建平谢锋
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S