Application of kaempferol as synergist of anti-fungal medicaments

A technology of antifungal drugs and synergists, applied in the field of medicine, to achieve the effects of reducing toxic side effects and treating fungal infections

Inactive Publication Date: 2011-10-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report that kaempferol can be used a

Method used

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  • Application of kaempferol as synergist of anti-fungal medicaments
  • Application of kaempferol as synergist of anti-fungal medicaments
  • Application of kaempferol as synergist of anti-fungal medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Effects of combined use of kaempferol and fluconazole on different clinical fungal strains.

[0011] Materials and methods

[0012] 1. Test drug:

[0013] Kaempferol: China Institute for the Control of Pharmaceutical and Biological Products (the same below).

[0014] Fluconazole: Pfizer Pharmaceutical Co., Ltd. (the same below).

[0015] Dimethyl sulfoxide: China Pharmaceutical (Group) Shanghai Chemical Reagent Company.

[0016] The concentration of kaempferol was 8 mg / ml, the concentration of fluconazole was 2 mg / ml, and the tested drugs were stored at -20°C. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0017] 2. Strains:

[0018] The clinical strains of Candida albicans, Candida krusei and Microsporum lanoides were provided by the fungal laboratory of Shanghai Changhai Hospital, and were identified by morphology and biochemi...

Embodiment 2

[0046] Embodiment 2: the effect of kaempferol combined with fluconazole on different clinical and laboratory induced drug-resistant strains

[0047] Materials and methods

[0048] 1. Test drug:

[0049] The sources of kaempferol and fluconazole are the same as above.

[0050] Dimethyl sulfoxide: China Pharmaceutical (Group) Shanghai Chemical Reagent Company.

[0051] The concentration of kaempferol was 8 mg / ml, the concentration of fluconazole was 2 mg / ml, and the tested drugs were stored at -20°C. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0052] 2. Strains:

[0053] Clinical drug-resistant strain: Candida albicans, provided by the Fungi Department of Shanghai Changhai Hospital, confirmed by morphological and biochemical identification.

[0054] Drug-resistant strains induced in the laboratory: Candida albicans (SC5314-R, Y01-R) are drug-resis...

Embodiment 3

[0062] Embodiment 3: the combination medicine of kaempferol and miconazole, ketoconazole

[0063] Materials and methods

[0064] 1. Test drug:

[0065] Kaempferol: National Institute for the Control of Pharmaceutical and Biological Products.

[0066] Fluconazole: Pfizer Pharmaceuticals Ltd.

[0067] Ketoconazole: National Institute for the Control of Pharmaceutical and Biological Products.

[0068] Miconazole: National Institute for the Control of Pharmaceutical and Biological Products.

[0069] Dimethyl sulfoxide: China Pharmaceutical (Group) Shanghai Chemical Reagent Company.

[0070] The concentration of kaempferol was 8 mg / ml, the concentration of miconazole and ketoconazole was 6.4 mg / ml, and the tested drugs were stored at -20°C. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0071] Other experimental steps and methods are the same as in E...

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Abstract

The invention relates to the technical field of medicaments, in particular to novel application of kaempferol as a synergist of anti-fungal medicaments. The anti-fungal medicaments are azole-type or polyene-type anti-fungal medicaments and based on the effective concentration of the anti-fungal medicaments, the adding ratio of the kaempferol is 0.5 to 16 mu g/ml. Tests show that when the kaempferol and the anti-fungal medicaments such as fluconazole, ketoconazole, miconazole and amphotericin B are used together, not only the anti-fungal effect is guaranteed on the premise of lowering the consumption of the anti-fungal medicaments, but also the anti-fungal medicaments can restore the function of killing the drug-resistant fungi, so that the kaempferol can be used as a synergist of the anti-fungal medicaments. The kaempferol used as the synergist of the anti-fungal medicaments can lower the consumption of the azole-type or polyene-type anti-fungal medicaments so as to reduce the toxic and side effects of the medicaments; and the kaempferol can make the anti-fungal medicaments restore the function of killing the drug-resistant fungi, so that the kaempferol can effectively treat mycotic infection, particularly drug-resistant t mycotic infection, and has quite important clinical application values.

Description

technical field [0001] The invention relates to the technical field of medicine, and is a new application of kaempferol as a synergist of antifungal drugs. Background technique [0002] Antifungal drugs such as azoles (including imidazoles and triazoles) and polyenes are commonly used clinically and can effectively treat deep and superficial fungal infections, such as ketoconazole and clotrimazol in imidazoles azole, miconazole, econazole, etc.; fluconazole, itraconazole, voriconazole, etc. in triazoles; amphotericin B in polyenes, etc. However, due to well-known reasons, clinical drug-resistant strains are becoming more and more common, resulting in the need to increase the dosage, and some antifungal drugs are ineffective even by increasing the dosage. Increasing the dosage will inevitably increase the toxic and side effects, especially drugs such as ketoconazole and other toxic side effects on the liver or heart, thereby limiting the clinical application of these drugs. ...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K31/4196A61K31/496A61K31/4174A61K31/7048A61P31/10
Inventor 曹颖瑛朱臻宇郑泽宇徐铮柴逸峰姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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