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Preparation method of cherokee rose polysaccharide derivatives with antitumor activity

A technology of polysaccharide derivatives and anti-tumor activity, which is applied in the fields of chemical modification of plant polysaccharides and preparation of anti-tumor drugs, and can solve the problems that the research on the anti-tumor biological activity of polysaccharide structure modification of jasper japonica has not been carried out.

Inactive Publication Date: 2011-10-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, preliminary research has been carried out on the polysaccharides of Rosa chinensis (RLP), and some research results have been obtained, mainly focusing on anti-oxidation, lipid-suppressing effects, immune activity, Antibacterial and anti-inflammatory effects, etc., but the research on the structure modification and anti-tumor biological activity of the polysaccharide of Rosa chinensis has not yet been carried out

Method used

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  • Preparation method of cherokee rose polysaccharide derivatives with antitumor activity
  • Preparation method of cherokee rose polysaccharide derivatives with antitumor activity
  • Preparation method of cherokee rose polysaccharide derivatives with antitumor activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Preparation of Polysaccharide

[0062] (1) as figure 1 First, crush the fruit of S. japonica; then reflux with petroleum ether at 50 °C for 1 hour to degreasing; then reflux with 80% wt ethanol at 96 °C for two times, each time for 2 hours, after leaching with 80 °C hot water for 2 hours, combine the extracts, It is then subjected to gauze filtration and Buchner funnel suction filtration; after concentration and concentration, anhydrous ethanol is added for precipitation, and then the precipitation is dried to obtain golden cherry crude polysaccharide;

[0063] (2) The crude polysaccharide of the golden cherry seeds was dissolved in 10mLH 2 O, 0.1 and 0.2mol / L NaCl solution, add DEAE-cellulose 52 (filler) for static adsorption for 30min, rinse DEAE-cellulose 52, filter the eluate, collect the filtrate to be the decolorized crude polysaccharide solution; detect DEAE-fiber The decolorization rate of the adsorption of element 52 in different solvents is shown i...

Embodiment 2

[0081] Example 2 Preparation of Fructus Rosa Fructus Sulfated Polysaccharide

[0082] (1) if figure 1 First crush the fruit of Rosa japonica; then degrease with petroleum ether at 50°C for 1 hour; then reflux twice with 80% wt ethanol at 96°C for 2 hours each time, extract with hot water at 80°C for 2 hours, combine the extracts, It is then subjected to gauze filtration and Buchner funnel suction filtration; after concentration and concentration, absolute ethanol is added to precipitate, and the precipitate is dried to obtain the crude polysaccharide of Rosa chinensis;

[0083] (2) Crude polysaccharides from Fructus Rosa Rosa were dissolved in 10mLH 2 O, add DEAE-cellulose 52 (filler) for static adsorption for 30 minutes, rinse the DEAE-cellulose 52, filter the eluate, and collect the filtrate to obtain the decolorized crude polysaccharide solution;

[0084] (3) Add papain to the decolorized crude polysaccharide solution, degrade it in a water bath at 60°C for 3 hours, adjus...

Embodiment 3

[0088] Example 3 Preparation of Carboxymethylated Polysaccharides of Rosa Fructus Rosa

[0089] Weigh 5 g of Fructus Rosa Rosa Polysaccharide prepared in Example 2 and place it in a reaction flask, add 76 mL of isopropanol, stir and soak for 30 min, add 25 mL of 10% NaOH solution, and soak at 30°C for 1 h. Dissolve 4g of chloroacetic acid in 10mL of isopropanol, add it to the above soaking solution, add 25mL of 10% NaOH solution, and react at 70°C for 2.5h. After inactivation, add four times the volume of 95% ethanol to precipitate the polysaccharide, add a small amount of water to dissolve, repeat alcohol precipitation and water dissolution for 3 times, concentrate, and dry to obtain the product carboxymethylated polysaccharide.

[0090] The polysaccharide carboxymethylation product of Rosa chinensis was purified by DEAE-52, and further purified by DEAE-Sepharose FF ion exchanger, and the elution peak shape was more symmetrical. The main peak polysaccharides were collected, ...

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Abstract

The invention discloses a preparation method of cherokee rose polysaccharide derivatives with an antitumor activity. The preparation method comprises the following steps: firstly smashing cherokee rose fruit, degreasing, removing oligosaccharide, lixiviating with hot water twice, then mixing extracts, filtrating, concentrating, precipitating and drying so as to obtain crude cherokee rose polysaccharide; adding diethylin ethyl amino ethyl (DEAE)-cellulose 52 to carry out static absorption for 30 minutes; washing with distilled water, filtering, and collecting filtrate so as to obtain the decolored crude cherokee rose polysaccharide; carrying out protein removal on a crude polysaccharide solution by using an enzyme method and a Sevag method; further separating the crude cherokee rose polysaccharide with the DEAE-cellulose 52, collecting, dialyzing, and freeze-drying so as to obtain cherokee rose polysaccharide; and finally, carrying out sulphating, carboxymethylation and hydrochloric acid degradation on the cherokee rose polysaccharide, and carrying out carboxymethylation on degradation products so as to obtain a plurality of cherokee rose polysaccharide derivatives. The plurality of cherokee rose polysaccharide derivatives have in vitro antitumor characteristics, and can be used for inhibiting the growth of three tumor cells, namely, ovarian cancer cells, liver cancer cells and rectal cancer cells.

Description

technical field [0001] The invention belongs to the technical field of bioactive polysaccharides, and mainly relates to the chemical modification of plant polysaccharides and the preparation method of antitumor drugs. Background technique [0002] Carbohydrates are an important class of biomolecules other than proteins and nucleic acids in organisms, especially an important class of information molecules, which have various biological functions. Among them, polysaccharides are abundant biopolymers in nature, which are polymers linked by aldose or ketose through glycosidic bonds, and widely exist in animal cell membranes, plant and microbial cell walls. Hundreds of active polysaccharides have been found so far, including fungal polysaccharides, higher plant polysaccharides, algal lichen polysaccharides, animal polysaccharides, and bacterial polysaccharides. Polysaccharides have multiple biological functions such as energy storage, structural support, defense and antigenic de...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 肖凯军刘晓红董浩
Owner SOUTH CHINA UNIV OF TECH
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