Supercharge Your Innovation With Domain-Expert AI Agents!

Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material

A technology of nanomaterials and tissue culture, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the unstudied problems of the influence of carbon nanotubes on the differentiation and growth of soybean cotyledonary node explants, and achieve organizational Improve the culture efficiency, the effect is obvious, and the effect of the high-efficiency tissue culture system

Inactive Publication Date: 2011-11-02
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
View PDF4 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] CN101822140 discloses the method of using carbon nanotubes to promote soybean seed germination under drought stress conditions, but does not study the influence of carbon nanotubes on soybean cotyledon node explant differentiation and growth, and seed germination and cotyledon differentiation and growth are two mechanisms different physiological processes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material
  • Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material
  • Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Seeds for testing: soybean variety Zhonghuang 35, 1500 grains.

[0024] Nanomaterials: carbon nanotubes (NM) (single wall)

[0025] Medium formula:

[0026] Seed germination medium: B5+ sucrose 20g / L+ agar 7.5g / L

[0027] Differentiation medium

[0028] SINM0: B5+6-BA1.50mg / L+ sucrose 30g / L+ agar 7.5g / L

[0029] SINM4: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​4mg / L

[0030] SINM40: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​40mg / L

[0031] SINM400: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​400mg / L

[0032] regeneration medium

[0033] SENM0:

[0034] B5+ZT1mg / L+IAA0.1mg / L+GA 3 0.5mg / L+L-pyrogltamic acid100mg / L+asparagines

[0035] 50mg / L+ sucrose 30g / L+ agar 7.5g / L

[0036] SENM4:

[0037] B5+ZT1mg / L+IAA0.1mg / L+GA 3 0.5mg / L+L-pyrogltamic acid100mg / L+asparagines

[0038] 50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​4mg / L

[0039] SENM40:

[0040] B5+ZT1mg / L+IAA0.1mg / L+GA 3 0.5mg / L+L-pyrogltamic acid100mg / L+asparagines

[0041] 50mg / L+sucrose 30g / ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of crop planting, and in particular relates to a tissue culture method for promoting differentiation and regeneration of a soybean cotyledon node explant by using a nano material. The method comprises the following steps: 1) disinfecting seeds; 2) germinating the seeds; 3) preparing a cotyledon explant; 4) putting the obtained explant on an induction medium containing a carbon nano tube at a concentration of 4-400mg / L to induce the explant to be differentiated; 5) transferring the differentiated soybean cotyledon node explant to an elongating and rooting medium containing a carbon nano tube at a concentration of 4-400mg / L; and 6) separating the tissue culture seedlings. The tissue culture method has the following beneficial effects: the method is easy tooperate and has an obvious effect; an efficient tissue culture system can be established; the tissue culture workload is not increased; and the method is of great importance to improvement of the soybean tissue culture efficiency.

Description

technical field [0001] The invention relates to the field of crop planting, in particular to a tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explants by using nanometer materials. Background technique [0002] Soybean is one of the six important crops in my country. In soybean production, it is often affected by adversity factors such as diseases, insect pests, weeds and drought, and the yield is very unstable; although conventional breeding techniques have played an important role in cultivating new varieties with high yield, stable yield, high quality and strong stress resistance However, due to the influence of species hybridization incompatibility and linkage with bad gene traits, some unwanted bad genes are also brought in while introducing good foreign genes, so it is difficult to further use. Genetic engineering technology has opened up a new way in this respect. It can break the boundaries of species and realize the r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 黄其满王晓红王岚王连铮
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com