Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material

A technology of nanomaterials and tissue culture, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve the unstudied problems of the influence of carbon nanotubes on the differentiation and growth of soybean cotyledonary node explants, and achieve organizational Improve the culture efficiency, the effect is obvious, and the effect of the high-efficiency tissue culture system

Inactive Publication Date: 2011-11-02
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] CN101822140 discloses the method of using carbon nanotubes to promote soybean seed germination under drought stress conditions, but does not study the influence of carbon nano

Method used

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  • Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material
  • Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material
  • Tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explant by using nano material

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] Test seed: Soybean variety Zhonghuang 35,1500 grains.

[0024] Nanomaterial: Carbon Nanotube (NM) (Single Wall)

[0025] Medium formula:

[0026] Seed germination medium: B5+sucrose 20g / L+agar 7.5g / L

[0027] Differentiation medium

[0028] SINM0: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L

[0029] SINM4: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​4mg / L

[0030] SINM40: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​40mg / L

[0031] SINM400: B5+6-BA1.50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​400mg / L

[0032] Regeneration medium

[0033] SENM0:

[0034] B5+ZT1mg / L+IAA0.1mg / L+GA 3 0.5mg / L+L-pyrogltamicacid100mg / L+asparagines

[0035] 50mg / L+sucrose 30g / L+agar 7.5g / L

[0036] SENM4:

[0037] B5+ZT1mg / L+IAA0.1mg / L+GA 3 0.5mg / L+L-pyrogltamicacid100mg / L+asparagines

[0038] 50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​4mg / L

[0039] SENM40:

[0040] B5+ZT1mg / L+IAA0.1mg / L+GA 3 0.5mg / L+L-pyrogltamicacid100mg / L+asparagines

[0041] 50mg / L+sucrose 30g / L+agar 7.5g / L+NM ​​40mg / L

[0042] SENM400:...

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Abstract

The invention relates to the field of crop planting, and in particular relates to a tissue culture method for promoting differentiation and regeneration of a soybean cotyledon node explant by using a nano material. The method comprises the following steps: 1) disinfecting seeds; 2) germinating the seeds; 3) preparing a cotyledon explant; 4) putting the obtained explant on an induction medium containing a carbon nano tube at a concentration of 4-400mg/L to induce the explant to be differentiated; 5) transferring the differentiated soybean cotyledon node explant to an elongating and rooting medium containing a carbon nano tube at a concentration of 4-400mg/L; and 6) separating the tissue culture seedlings. The tissue culture method has the following beneficial effects: the method is easy tooperate and has an obvious effect; an efficient tissue culture system can be established; the tissue culture workload is not increased; and the method is of great importance to improvement of the soybean tissue culture efficiency.

Description

technical field [0001] The invention relates to the field of crop planting, in particular to a tissue culture method for promoting differentiation and regeneration of soybean cotyledon node explants by using nanometer materials. Background technique [0002] Soybean is one of the six important crops in my country. In soybean production, it is often affected by adversity factors such as diseases, insect pests, weeds and drought, and the yield is very unstable; although conventional breeding techniques have played an important role in cultivating new varieties with high yield, stable yield, high quality and strong stress resistance However, due to the influence of species hybridization incompatibility and linkage with bad gene traits, some unwanted bad genes are also brought in while introducing good foreign genes, so it is difficult to further use. Genetic engineering technology has opened up a new way in this respect. It can break the boundaries of species and realize the r...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄其满王晓红王岚王连铮
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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