Method for extracting general flavone from herba desmodii styracifolii

A technology of Desmodium sativa and total flavonoids, which is applied in the field of natural product separation, to achieve the effect of increasing the processing capacity, reducing the adsorption amount, and reducing interference

Inactive Publication Date: 2011-11-09
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the literature "Study on Extraction Technology of Total Flavonoids from Desmodium sativa", the best technology determined in this literature is: 15 times the amount of water reflux extraction 3 times, each time 1.5h, this method also does not further purify the total flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Take 1 kg of Desmodium herb, crush it, add it to the extraction kettle, and use 12L of 50% methanol to reflux for extraction. The extract is placed in a vacuum concentration kettle, concentrated to 1 / 2 of the original volume, left to stand, and the supernatant is separated.

[0019] 2) Dilute the above supernatant with water, pass through an ultrafiltration membrane for ultrafiltration, the ultrafiltration membrane has a pore size of 5 nm, and a permeation pressure of 0.5 MPa to remove water-soluble macromolecules to obtain an ultrafiltration treatment solution.

[0020] 3) Take the AB-8 type macroporous resin for wet packing, after washing with water to balance, slowly pass the above ultrafiltration treatment solution into the resin column, control the adsorption flow rate to 5ml / min, first elute with 3BV water, and then use 4BV80 % ethanol solution for elution, combined eluents, and concentrated to obtain 8.5 g of total flavonoids of Desmodium stylifolia.

Embodiment 2

[0022] 1) Take 1kg of Desmodium herb, crush it, add it to the extraction kettle, and use 18L of 70% ethanol to reflux for extraction. The extract is placed in a vacuum concentration kettle, concentrated to 2 / 3 of the original volume, left to stand, and the supernatant is separated.

[0023] 2) Dilute the above supernatant with water, pass through an ultrafiltration membrane for ultrafiltration, the ultrafiltration membrane has a pore size of 10 nm, and a permeation pressure of 0.3 MPa to remove water-soluble macromolecules to obtain an ultrafiltration treatment solution.

[0024] 3) Take D001 type macroporous resin for wet packing, after washing with water to balance, slowly pass the above ultrafiltration treatment solution into the resin column, control the adsorption flow rate to 6ml / min, first elute with 1BV of water, and then use 5BV of 75% ethanol respectively The solution was eluted, and the eluents were combined and concentrated to obtain 9.1 g of total flavonoids of Des...

Embodiment 3

[0026] 1) Take 5kg of Desmodium herb, crush it, add it to the extraction kettle, use 75L of 60% isopropanol to reflux extract, place the extract in a vacuum concentration kettle, concentrate to 2 / 3 of the original volume, let it stand, and separate the supernatant .

[0027] 2) Dilute the above supernatant with water, pass through an ultrafiltration membrane for ultrafiltration, the ultrafiltration membrane has a pore size of 5 nm, and a permeation pressure of 0.3 MPa to remove water-soluble macromolecules to obtain an ultrafiltration treatment solution.

[0028] 3) Take the AB-8 type macroporous resin and pack it into a column by wet method. After washing with water to balance, slowly pass the above ultrafiltration treatment solution into the resin column, control the adsorption flow rate to 10ml / min, first elute with 2BV water, and then use 7BV80 % ethanol solution for elution, combined eluents, and concentrated to obtain 35.4 g of total flavonoids of Desmodium stylifolia. ...

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Abstract

The invention relates to a method for extracting general flavone from herba desmodii styracifolii, in particular to a preparation process for extracting the purified general flavone from the herba desmodii styracifolii. The method comprises the following process steps of: taking herba desmodii styracifolii medicinal materials, adding low-carbon alcohols into the herba desmodii styracifolii medicinal materials for reflowing and extracting; condensing an extraction solution to be small volume; standing and removing precipitation; removing water-soluble macromolecules by using an ultrafiltration membrane; feeding macroporous resin and adsorbing; and eluting with an alcohol solution and collecting the general flavone of the herba desmodii styracifoli. The method has the advantages of simpleness in operation, low requirement for equipment, higher purity of the obtained product, and the like.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting total flavonoids from Desmodium sativa, especially a method for purifying total flavonoids of Desmodium sativa by using membrane separation combined with a macroporous resin. Background technique: [0002] Desmodium styracifolium (Osbeck) Merr. is a leguminous plant of the genus Osbeck. It is abundant in Guangxi, Guangdong, Fujian and other places. Its nature and flavor are sweet, light and cool. It is a traditional Chinese medicine commonly used in my country. The function of diuresis and stone expulsion is used for urinary system infection, urinary system stones, cholelithiasis, acute icteric hepatitis, etc., containing flavonoids, alkaloids, saponins, phenols and other ingredients. Pharmacological studies have proved that the flavonoid glycosides in Desmodium sativa can prevent the formation of calcium oxalate urolithiasis in ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P13/04A61P9/00A61P25/00
Inventor 刘东锋吴艳波杨成东
Owner NANJING ZELANG MEDICAL TECH
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