Process for preparing high-purity resveratrol from giant knotweed

Abstract

The invention discloses a method for preparing high-purity resveratrol from giant knotweed. The method comprises the following steps of: smashing dry giant knotweed till 90 percent of smashed giant knotweed can pass through a 80-mesh screen, adding special microorganisms to water-soaked giant knotweed powder, fermenting at constant temperature 35-60 DEG C for 12-30 hours, adding low-temperature yeast, lowering the temperature to 20-30 DEG C and fermenting at constant temperature for 30-48 hours; extracting with ethanol, concentrating the extracting solution and extracting the concentrated solution with ethyl acetate or butyl acetate in the ratio 1:1; and feeding the obtained organic layer into an alumina chromatography column, eluting with 80-90% ethanol, concentrating the eluent till thealcohol concentration is 40-60 percent, crystallizing, recrystallizing, standing for 5-8 hours, filtering and recrystallizing till crystals are white. By adopting the method, the purity of resveratrol can be increased to over 99 percent, the product yield is increased from 0.3-0.5 percent to over 0.8 percent, the color of a resveratrol product is white without any mixed color, the product qualityis enhanced, the production period can be shortened, and the production efficiency is increased.

Description

technical field [0001] The invention relates to the field of extracting effective plant components, in particular, it provides a process for preparing resveratrol from Polygonum cuspidatum. Background technique [0002] At present, the classic process route for domestic production enterprises to produce resveratrol is: Polygonum cuspidatum—fermentation—alcohol extraction and concentration—dry column chromatography (silica gel or alumina)—activated carbon decolorization—crystallization and recrystallization. The yield of the final product resveratrol in this process route is generally only 0.3-0.5%, and the color is slightly beige or off-white. The purity of resveratrol is 95-98%. Its price and purity are 99% There is a big difference between retrol. The purity of resveratrol obtained by the existing extraction method is quite different from the resveratrol content of Polygonum cuspidatum. How to improve the yield, whiten the product color and improve product quality is a p...

AI Technical Summary

Problems solved by technology

At present, the production technology of more than 99% resveratrol has not been reported in the literature.