Off-Gel free flow electrophoresis coupling chip and making method thereof
An off-gel and electrophoresis chip technology, which is applied in the field of microfluidic chip processing and production, can solve the problems of poor reproducibility and achieve the effect of improving separation performance and simple production
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[0016] The invention will be described in further detail below in conjunction with the accompanying drawings.
[0017] like figure 1 , 2 As shown, the Off-Gel free-flow electrophoresis coupling chip of the present invention includes a chip substrate (1), an electrode (2), and a separation chamber (3), and contains a fixed pH gradient strip region at the end of the separation chamber (3) (4) and a hard substrate (5) for fixing the embedding and immobilizing the pH gradient gel.
[0018] Conventional free-flow electrophoresis chips are usually composed of two substrates combined and sealed, and an electrode area and a separation chamber area are formed in the inner cavity. We have carried out structural transformation on the separation chamber area of one of the substrates, and opened a A rectangular area for embedding the fixed pH gradient strip, the length and width of which are smaller than the fixed pH gradient strip. Align the modified chip substrate with another subst...
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