System and method for surface plasmon resonance sensing detection

Abstract

The invention which is suitable for a photoelectric detection technology provides a system and a method for surface plasmon resonance sensing detection. The system comprises: light sources for generating sensing lights; a first lens for adjusting the sensing lights into collimated lights; a polarizer for obtaining P-polarized lights in the collimated lights; a prism for receiving the P-polarized lights to irradiate the P-polarized lights onto a sensing surface; a controller for controlling the light sources to allow the light sources to be sequentially lightened at different positions of a focal plane of the first lens and sequentially irradiate each P-polarized light onto the sensing surface from different angles; and an imaging mechanism for recording the intensity distribution of lights reflected by the sensing surface. According to the invention, the light sources are sequentially lightened at different positions of the focal plane of the first lens through an electronic control mode, so the P-polarized lights are sequentially irradiated onto the sensing surface from different angles, the intensity distribution of the lights reflected by the sensing surface is recorded in real time, SPR angular scan curves of each point on the sensing surface are obtained in parallel, the stability is good, and the speed is fast.

Description

technical field [0001] The invention belongs to photoelectric detection technology, in particular to a surface plasmon resonance sensing detection system and method. Background technique [0002] Surface plasmon resonance (Surface Plasmon Resonance, SPR) is an emerging sensing technology, which has the advantages of high sensitivity, high throughput, easy to achieve specific detection and real-time, and does not require labeling, etc., and has been widely used in biological , medicine, food quality and safety, chemical and environmental monitoring and other industries, especially for online real-time detection of biomolecules such as DNA and protein, protein molecules, and drug-protein, nucleic acid-nucleic acid, antigen-antibody, receptor-ligand, etc. interactions between them, etc. However, the current SPR sensing technology requires a mechanical scanning device to modulate the angle of the light source, which has poor stability and low precision. Contents of the invent...

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Problems solved by technology

[0003] The purpose of the embodiments of the present invention is to provide a surface plasmon resonance intensity detection system, which aims to solve the problem that the existing surface plasmon resonance intensity detection system needs to use a mechanical scanning device to modulate the angle of the light source and has poor stability.

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