Bacillus pumilus and application thereof on killing eelworms

A technology of bacillus pumilus and whole tooth resurrection of nematodes, applied in the direction of nematicides, applications, bacteria, etc., to achieve good killing effect

Inactive Publication Date: 2014-08-20
HONGYUN HONGHE TOBACCO (GRP) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have not been any reports of Bacillus pumilus killing root-knot nematodes

Method used

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  • Bacillus pumilus and application thereof on killing eelworms
  • Bacillus pumilus and application thereof on killing eelworms
  • Bacillus pumilus and application thereof on killing eelworms

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Embodiment Construction

[0027] 1. Bacillus pumilus ( Bacillus pumilus ) preparation

[0028] Bacillus pumilus ( Bacillus pumilus ) obtained from the wild, the specific cultivation method is as follows:

[0029] 1) Test tube culture: beef extract peptone medium: peptone 10g, beef extract 3g, sodium chloride 5g, agar 13g, distilled water 1000ml, pH6.8~7.2, inoculate Bacillus pumilus on the medium, 25℃~ Cultivate at 37°C for 24 hours to obtain test tube seeds;

[0030] 2) Liquid expansion culture: The formula of liquid medium is: every 1000ml of distilled water contains 3g of beef extract, 10g of peptone, pH7.2~7.4, inoculate the test tube species of Bacillus pumilus into 250ml of Erlenmeyer flask liquid medium, each bottle contains 100ml, Cultivate on a shaking table at 25°C-37°C for 24 hours, with a rotation speed of 150-250rpm.

[0031] 2. Bacillus pumilus ( Bacillus pumilus ) nematicide activity test

[0032] 1) Preparation of whole-tooth revived nematodes ( Panagrellus redivivus ) nematodes...

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Abstract

The invention relates to a bacillus pumilus and its application in killing nematodes. The morphological characteristics of the Bacillus pumilus strain with nematicidal function used in the present invention are short rod-shaped, bacterium with a size of 0.58×2.1um, endophytic spores, and Gram staining is positive. On beef extract peptone solid medium, the colonies are protruding, with smooth and fine edges, milky white, and a diameter of 1.1mm to 1.8mm; on DSMZ88 improved solid medium, the colonies are smaller and white, with dry surface, rough edges and irregularities , the diameter is 1.5-2.0mm. The present invention is prepared by conventional methods. That is to say, after the Bacillus pumilus is expanded and cultivated, its insecticidal effect on Panagrellus redivivus, Meloidogyneincognita and root-knot nematode eggs is determined. The Bacillus pumilus of the present invention has good killing effect on P.redivivus nematodes, root-knot nematodes and root-knot nematode eggs, and can be developed as a nematode biocontrol agent.

Description

technical field [0001] The present invention relates to the technical field of microbial pesticides, specifically a kind of bacillus pumilus ( Bacillus pumilus ) application in killing nematodes. Background technique [0002] Plant parasitic nematodes are a common plant disease worldwide. There are more than 70 known species of root-knot nematodes alone, which harm more than 3,000 plants and cause huge losses every year. Chemical nematicides have played an important role in nematode control since their discovery in the 1940s. With the advancement of science and technology, many chemical nematicides have been found to have side effects, causing environmental pollution and endangering human health. Many have been disabled or will be disabled one after another. In particular, plant parasitic nematodes occur in the roots of crops. Due to the particularity of the soil environment, it must be applied in large doses to ensure its control effect. However, large doses of chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00A01P5/00C12N1/20A01K67/033C12R1/01
CPCY02A40/22
Inventor 徐洁常寿荣王绍坤罗华元陶南朱海滨
Owner HONGYUN HONGHE TOBACCO (GRP) CO LTD
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