Compound probiotic microcapsules that can improve the intestinal microecology of small dogs
A technology of compound probiotics and intestinal microecology, applied in application, animal feed, animal feed, etc., can solve the problems of short shelf life and substandard viable bacteria concentration.
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[0011] The present invention will be described in detail below in conjunction with specific examples.
[0012] The medium used in the present invention is conventional medium or recommended medium.
[0013] (1) First, Lactobacillus acidophilus (Lactobacillus acidophilus, purchased from CICC, preservation number 6075), plant Lactobacillus plantarum (Lactobacillus plantarum, purchased from CICC, preservation number 22696) and rhamnosus Lactobacillus (Lactobacillus rhamnosus, purchased from CICC, deposit number 6135) After strain passage and strain compound induction screening, the order of speech is adjusted here. After culturing on nutrient agar medium at 35-40°C for 35-45 hours, store them in a refrigerator at 2-4°C for later use; weigh the above three strains according to the weight ratio of 2:1:2, and inoculate with 3-5% of the inoculum Adjust the pH value to 6.5-7.3 in the steam-sterilized culture medium, put it into a fermenter for cultivation and fermentation for 15-20 h...
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