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Compound probiotic microcapsules that can improve the intestinal microecology of small dogs

A technology of compound probiotics and intestinal microecology, applied in application, animal feed, animal feed, etc., can solve the problems of short shelf life and substandard viable bacteria concentration.

Inactive Publication Date: 2011-12-21
XIHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing probiotic products have problems such as short shelf life and substandard concentration of viable bacteria.

Method used

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  • Compound probiotic microcapsules that can improve the intestinal microecology of small dogs

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Embodiment Construction

[0011] The present invention will be described in detail below in conjunction with specific examples.

[0012] The medium used in the present invention is conventional medium or recommended medium.

[0013] (1) First, Lactobacillus acidophilus (Lactobacillus acidophilus, purchased from CICC, preservation number 6075), plant Lactobacillus plantarum (Lactobacillus plantarum, purchased from CICC, preservation number 22696) and rhamnosus Lactobacillus (Lactobacillus rhamnosus, purchased from CICC, deposit number 6135) After strain passage and strain compound induction screening, the order of speech is adjusted here. After culturing on nutrient agar medium at 35-40°C for 35-45 hours, store them in a refrigerator at 2-4°C for later use; weigh the above three strains according to the weight ratio of 2:1:2, and inoculate with 3-5% of the inoculum Adjust the pH value to 6.5-7.3 in the steam-sterilized culture medium, put it into a fermenter for cultivation and fermentation for 15-20 h...

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Abstract

The invention discloses a composite probiotic microcapsule capable of improving the intestinal microecology of a small dog. The preparation method comprises the following steps: A1, preparing a composite bacterial preparation; A2, preparing a composite probiotic bacteria liquid; A3, mixing 2-5% The porous starch was mixed with the composite probiotic liquid, the pH value was adjusted to 6.0, and the temperature was controlled at 25-35°C for 30 minutes of shaking. After being fully adsorbed, add the following outer layer embedding materials in sequence according to the weight percentage: 1-3% sodium alginate, 1-5% gelatin, 1-5% dextrin, stir evenly, drop calcium chloride solution to solidify, freeze drying to obtain composite probiotic microcapsules. The invention uses porous starch as the core material, absorbs the composite probiotic bacteria liquid, and then uses sodium alginate, dextrin and gelatin as the wall materials, and processes to obtain the composite probiotic bacteria microcapsules.

Description

technical field [0001] The invention relates to a compound probiotic microcapsule capable of improving the intestinal microecology of small dogs, in particular to a compound probiotic microcapsule embedded in porous starch and capable of improving the intestinal microecology of small dogs. Background technique [0002] In my country, with the improvement of people's living standards, pet owners are paying more and more attention to the quality of life of their pets. After the initial blind breeding, more and more pet owners began to pay attention to the health problems of their pets, and various pet health care products also entered the pet family. However, due to the late start of research in this industry in China, most of the domestic probiotic preparations specially used for pet dogs, especially small dogs, are single bacterial preparations. [0003] Probiotics are live microorganisms that work by improving the balance of the host's gut microbiota. Oral administration ...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A23K10/18A23K50/42
Inventor 马嫄车振明陈祥贵李玉锋芮光伟杨潇朱胜华
Owner XIHUA UNIV
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