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A kind of method adopting biological enzyme technology to prepare snake whip active peptide

A technology of active peptides and biological enzymes, applied in the field of biopharmaceuticals, can solve problems such as difficult to fully digest and efficiently utilize, and cannot change the structure of snake whip macromolecular proteins

Inactive Publication Date: 2011-12-21
唐作安
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The macromolecular protein structure of snake whip cannot be changed, and it is difficult to be fully digested and efficiently utilized by the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Select high-quality snake whips from adult healthy male individuals of black snake, pit viper, red snake, corona snake, and cobra; after cleaning, mincing, refining, breaking the wall, diluting, and heating; add 537 acid protease and Alcalase base Protease, Protamex compound protease, enzymatic hydrolysis for 4-8 hours, deactivation, centrifugal filtration; select cellulose acetate nanofiltration membrane with a molecular weight cut-off of 100-300Dalton for desalination, concentration, and filter with an ultrafiltration membrane with a molecular weight cut-off of 200-5000Dalton Purification; drying with a microwave vacuum dryer at 20-50°C to obtain dry powder of the active peptide of snake penis.

Embodiment 2

[0009] Select the active peptide dry powder obtained in Example 1, and directly use the capsule filling machine to make hard capsule products; or add wolfberry, eucommia, yam, kudzu root, schisandra, eleuthero, epimedium, dodder, cistanche to the dry powder of active peptide , saffron and other traditional Chinese medicine extracts, made into a series of capsule products, etc.

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PUM

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Abstract

Through the breakthrough of processing technology, the invention improves the bioavailability of snake whip, a rare and precious resource. Due to the use of biological enzyme technology, the indigestion and utilization characteristics of large molecular weight proteins have been fundamentally changed. The enzymatic hydrolysis of snake whip into active peptides is very beneficial to the human body's absorption and utilization, which greatly broadens its application range. Its technical scheme is: select high-quality snake whip, wash, mince, grind, break wall, enzymolysis, inactivate enzyme, filter, desalt by nanofiltration, ultrafiltration purification, microwave vacuum drying (or freeze-drying) to obtain snake whip activity. Peptide powder, more than 85% of its molecular weight is distributed between 200-5000 Dalton.

Description

technical field [0001] The invention relates to a method for preparing snake whip active peptide by adopting biological enzyme technology, which belongs to the field of biopharmaceuticals. Background technique [0002] In the current market, Shebian is usually used to make products such as Shebian Wine, Shebian Pills, and Shebian Capsules. What is used are simple techniques and original methods, such as high-temperature sterilization, high-temperature drying, mechanical crushing, liquor soaking and so on. The macromolecular protein structure of snake penis cannot be changed, and it is difficult to be fully digested and efficiently utilized by the human body. Therefore, improving the bioavailability of snake penis, a scarce and valuable resource, and seeking new breakthroughs in processing technology have become important scientific research topics. The method we want to explore must also be able to effectively retain the original nutritional components and biologically act...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 唐作安
Owner 唐作安
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