Method for producing feed additives by staged enzymatic hydrolysis of four-cornered clam processing waste
A feed additive and the technology of four-cornered clam, which is applied in the field of marine bioengineering enzymatic production technology, can solve the problems of waste of biological resources and easy pollution of the environment, and achieve pollution reduction, good food-attracting effect and growth-promoting effect, and rich amino acid content Effect
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Embodiment 1
[0025] The raw material Sijiao clam processing waste (the mixture of the Sijiao clam processing waste water and the remaining mantle, viscera and other soft substances on the inner surface of the shell, wherein the dry matter weight content is 15%) is put into the enzymatic hydrolysis tank for heating and sterilization ( 100°C, 15 minutes), add papain (the amount of enzyme added is 700u / g raw material) for mixing, adjust the pH to 7.2 with NaOH solution (please specify the concentration range), enzymolysis at 56°C for 7 hours, and heat the hydrolyzate at 85°C Inactivate the enzyme for 15 minutes, then add Flavourzyme endopeptidase and telopeptidase (the total enzyme addition amount of the two is 300u / g raw material, and the ratio of endopeptidase and telopeptidase is 1: 1) to mix, and use NaOH solution ( Please clarify the concentration range) Adjust the pH to 6.4, and continue the enzymatic hydrolysis at 50°C for 4 hours. Finally, raise the temperature of the enzymatic hydrol...
Embodiment 2
[0028] Put the waste from raw clam processing (wastewater from clam processing and soft substances such as mantle and viscera on the inner surface of the shell, wherein the dry matter weight content is 12%) into an enzymatic tank for heating and sterilization (100°C , 20 minutes), add papain (the amount of enzyme added is 700u / g raw material) for mixing, use NaOH solution (please specify the concentration range) to adjust the pH to 7.5, enzymolysis at 60°C for 5 hours, and heat the hydrolyzate for 80 Inactivate the enzyme at ℃ for 20 minutes, then add Flavourzyme endopeptidase and telopeptidase (the total enzyme addition amount of the two is 300u / g raw material, the ratio of endopeptidase and telopeptidase is 1:1) to mix, and use NaOH solution (Please specify the concentration range) Adjust the pH to 6.6 and continue the enzymatic hydrolysis for 5 hours at a temperature of 55°C. Finally, heat up the enzymolysis solution to 85°C to inactivate the enzyme for 15 minutes.
[0029...
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